For cryosections, larvae were processed as described.61 (link) Larvae were cut as 16 μm sections on a Leica CSM1816 cryostat. For in situ hybridization, sections were hybridized as described62 (link) with published antisense probes against collagen 2a1a63 (link) or collagen 10a1a.64 (link) Immunohistochemistry was performed as described62 (link) without the proteinase K step, with mouse anti-PCNA (Proliferating Cell Nuclear Antigen; 1/500; Santa Cruz Biotech; PC10), Alexa Fluor 488 conjugated Donkey anti-mouse secondary antibody (1/100 Jackson Immunoresearch 715-545-150) and DAPI (1:2000 Life Technologies D1306). Fluorescence imaging was conducted on a Leica SP8 confocal microscope with a 63X oil immersion objective.
Mouse anti pcna proliferating cell nuclear antigen
Manufactured by Santa Cruz Biotechnology
Mouse anti-PCNA (Proliferating Cell Nuclear Antigen) is a primary antibody product used for the detection of PCNA, a protein involved in DNA replication and cell cycle regulation. This antibody can be utilized in various immunological techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to identify and localize PCNA within biological samples.
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Lab products found in correlation
Alexa fluor 488 conjugated donkey anti mouse secondary antibody, by Jackson ImmunoResearch (1 mentions)
Csm1816 cryostat, by Leica (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Sp8 confocal microscope, by Leica (1 mentions)
Biotinylated anti mouse secondary antibody, by Vector Laboratories (1 mentions)
Abc complex, by Agilent Technologies (1 mentions)
Alexa fluor 568 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse anti pcna proliferating cell nuclear antigen
1
Cryosectioning and In Situ Hybridization of Larval Samples
2
Immunofluorescence and Immunohistochemistry for Liver Cells
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Liv2-sorted GPSC derived hepatocytes were incubated with rabbit anti-Ck18, -Dlk1, -albumin, -Ki67, -e-cadherin antibodies (Abcam) overnight at 4°C and according to the manufacturer’s instructions. Alexa Fluor 568-conjugated goat anti-rabbit IgG (Invitrogen) was used as secondary antibody. DAPI was used to stain nuclei. The samples were examined with a Zeiss microscope (Apotome software). Mice livers were fixed in formalin and embedded in paraffin. The liver sections were stained with mouse anti-PCNA (proliferating cell nuclear antigen) (Santa Cruz Biotechnology) using the M.O.M. (mouse on mouse) kit and revealed using biotinylated anti-mouse secondary antibody (Vector Laboratories) and the ABC complex (DAKO). Staining of teratoma sections with anti-Liv2 antibodies was performed as described above.
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