Cholesterol and 7KCh were purchased from Steraloids, Inc (Newport, RI). Hydroxypropyl-β-cyclodextrin (HPBCD) was purchased from Sigma-Aldrich (St. Louis, MO). HPBCD-7KCh solutions were prepared as previously described19 (link) to generate a stock of 10 mM 7KCh solution in 45% HPBCD. In all experiments involving the use of 7KCh, 7KCh was administetered in HPBCD. MCP1 recombination protein was purchased from R&D (Minneapolis, MN).
Hydroxypropyl β cyclodextrin hpbcd
Manufactured by Merck Group
Sourced in Germany
Hydroxypropyl-β-cyclodextrin (HPBCD) is a modified form of β-cyclodextrin, a cyclic oligosaccharide composed of seven glucose units. HPBCD is used as a pharmaceutical excipient to enhance the solubility, stability, and bioavailability of various drug compounds.
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Lab products found in correlation
3 protocols using hydroxypropyl β cyclodextrin hpbcd
1
Preparation of 7-Ketocholesterol in HPBCD
2
Ruxolitinib and JQ1 Compound Preparation
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Ruxolitinib and CHZ868 were provided by Novartis. (+)-JQ1 (JQ1) and iBET-151 were kindly provided by James Bradner (Dana Farber Cancer Institute, Boston, MA). For in vitro use, compounds were dissolved in dimethylsulfoxide (DMSO) (Sigma-Aldrich) at a final stock concentration of 10 mM (stored at −20°C) and diluted to the appropriate concentration from stock prior to use. For in vivo application, Ruxolitinib was dissolved and administered orally (oral gavage) in 0.5% methylcellulose solution (Sigma-Aldrich) at 90 mg/kg twice daily for the indicated duration. JQ1 was reconstituted in one part DMSO to nine parts 10% (w/v) hydroxypropyl-β-cyclodextrin (HPBCD) (Sigma-Aldrich) solution and administered via intraperitoneal injection at 50 mg/kg once daily for the indicated duration.
3
Creosote-Contaminated Soil Remediation
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Soil was collected from an antique facility located in Andújar (South-eastern Spain: 38°01'39''N, 4°03'58''W), where railway crossbar were treated with creosote and inorganic wood preservatives for almost 100 years. The soil was sampled from the top 0-20 cm layer, air-dried and crushed to pass through a 2-mm mesh sieve before analysis. The soil has not received any remediation treatment since this activity was abandoned. The soil was analysed for pH in a water:soil extract at the ratio 2.5:1, total carbonate content, particle size distribution, organic matter (OM) content and PAHs and PTEs contents. Detailed soil description is reported elsewhere (Madrid et al., 2019) (link).
General properties are: pH 8.14, 8.11 % OM, 23.2 % CaCO 3 ; 74.8 % sand, 14.8 % silt and 10.4 % clay (sandy loam texture).
Hydroxypropyl-β-cyclodextrin (HPBCD) was supplied by Sigma-Aldrich (Germany).
The rhamnolipid JBR-425 is a commercial mixture of mono-and di-rhamnolipids supplied by Jeneil Biosurfactant (USA). JBR-425 has been already described in detail elsewhere (Ozdemir and Malayoglu, 2004) (link).
PAHs and PTEs analytical standards were supplied by Sigma Aldrich and VWR International (Spain) respectively. Other chemical reagents used were always analytical grade quality.
General properties are: pH 8.14, 8.11 % OM, 23.2 % CaCO 3 ; 74.8 % sand, 14.8 % silt and 10.4 % clay (sandy loam texture).
Hydroxypropyl-β-cyclodextrin (HPBCD) was supplied by Sigma-Aldrich (Germany).
The rhamnolipid JBR-425 is a commercial mixture of mono-and di-rhamnolipids supplied by Jeneil Biosurfactant (USA). JBR-425 has been already described in detail elsewhere (Ozdemir and Malayoglu, 2004) (link).
PAHs and PTEs analytical standards were supplied by Sigma Aldrich and VWR International (Spain) respectively. Other chemical reagents used were always analytical grade quality.
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