Exosome samples were diluted in PBS (phosphate buffered saline) 1:10–1:100 and analysed using the Flow Nano Analyzer (NanoFCM Inc. (Nottingham, UK)), according to the manufacturer’s protocol [21 (link)]. Briefly, lasers were calibrated using 200 nm control beads (NanoFCM Ltd.), which were analysed as a reference for particle concentration. A mixture of various-sized beads (NanoFCM Inc.) was analysed to set a reference for size distribution. PBS was analysed as a background signal. Particle concentrations and size distributions were calculated using NanoFCM software (NanoFCM profession V1.0) and normalised to the cell number and dilution necessary for adequate NanoFCM reading.
200 nm control beads
Manufactured by NanoFCM
Sourced in China
The 200 nm control beads are a laboratory tool designed for size calibration and verification purposes. These beads have a nominal diameter of 200 nanometers and are intended to be used as a reference standard to ensure the accuracy and precision of particle size measurement instruments.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using 200 nm control beads
1
Exosome Characterization using Flow Nano Analyzer
2
Exosome Characterization by Flow Nano Analyzer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Exosome samples (10 μl) were diluted (1:100) and analyzed using the Flow Nano
Analyzer (NanoFCM Inc. China), according to the manufacturer’s protocol22 (link). Briefly, calibration of the instrument was performed using 200 nm
control beads (NanoFCM Inc. China), which were analyzed as a reference for
particle concentration. Furthermore, a mixture of beads with different sizes
(NanoFCM Inc. China) was provided as a reference for size distribution. PBS was
used as a background signal and subtracted from other measured values during
analysis. The number of particles after the sample was diluted was in the
optimal range of 4000–14,000. Particle concentration and size distribution were
analyzed using the NanoFCM software (NanoFCM Profession V1.0) and normalized for
the starting volume of exosome resuspension and the dilution factor that was
necessary for proper NanoFCM reading.
Analyzer (NanoFCM Inc. China), according to the manufacturer’s protocol22 (link). Briefly, calibration of the instrument was performed using 200 nm
control beads (NanoFCM Inc. China), which were analyzed as a reference for
particle concentration. Furthermore, a mixture of beads with different sizes
(NanoFCM Inc. China) was provided as a reference for size distribution. PBS was
used as a background signal and subtracted from other measured values during
analysis. The number of particles after the sample was diluted was in the
optimal range of 4000–14,000. Particle concentration and size distribution were
analyzed using the NanoFCM software (NanoFCM Profession V1.0) and normalized for
the starting volume of exosome resuspension and the dilution factor that was
necessary for proper NanoFCM reading.
3
Exosome Size and Concentration Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Exosome samples were diluted 1:100 and analyzed using the Flow Nano Analyzer (NanoFCM Inc.) according to manufacturer’s protocol. Briefy, the lasers were calibrated using 200 nm control beads (NanoFCM Inc.), which were then analyzed as a reference for particle concentration. Additionally, a mixture of diferent sized beads (NanoFCM Inc.) was analyzed to set reference for size distribution.
4
Exosome Analysis by Flow Nano Analyzer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Exosome samples were diluted 1:100 and analyzed using the Flow Nano Analyzer (NanoFCM Inc.) according to manufacturer's protocol. Briefy, the lasers were calibrated using 200 nm control beads (NanoFCM Inc.), which were then analyzed as a reference for particle concentration. Additionally, a mixture of diferent sized beads (NanoFCM Inc.) was analyzed to set reference for size distribution.
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