Clarus 500 gc ms

Manufactured by PerkinElmer

Sourced in United States

The Clarus 500 GC-MS is a gas chromatography-mass spectrometry (GC-MS) system designed for analytical applications. It features a capillary gas chromatograph and a mass spectrometer detector. The system is capable of separating and identifying chemical compounds in complex mixtures.

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Lab products found in correlation

Turbomass 5, by PerkinElmer (2 mentions) Dvb car pdms fiber, by Merck Group (2 mentions) α pinene, by Merck Group (1 mentions) β pinene, by Merck Group (1 mentions) Turbomass quadrupole mass spectrometer, by PerkinElmer (1 mentions) Chemstation software, by Agilent Technologies (1 mentions) Vf 5m, by Agilent Technologies (1 mentions)

9 protocols using clarus 500 gc ms

Comprehensive GC-MS Analysis of Essential Oil

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For the identification of the compounds, gas chromatography coupled to mass spectrometry (GC–MS) was performed using a Clarus 500 GC–MS from Perkin-Elmer Inc. apparatus equipped with the same capillary column, carrier, and operating conditions as described above for GC analysis. The ionization source temperature was set at 200 °C and an electron impact mode of 70 eV was employed. MS spectra were obtained by means of total ion scan (TIC) mode (mass range m/z 45–500 uma). The total ion chromatograms and mass spectra were processed with the Turbomass 5.4 software (Perkin-Elmer Inc.). Retention indexes were determined by injection of C₈–C₃₂ n-alkanes standard under the same conditions.
The EO components were identified by comparison of their mass spectra with those of the computer library NIST MS Search 2.0 and available data in the literature [61 ]. Identification of the following compounds was confirmed by comparison of their experimental RI with those of authentic reference standards (Sigma-Aldrich, Darmstadt, Germany): α-pinene, β-pinene, camphene, myrcene, limonene, (Z)-β-ocimene, camphor, borneol, terpinen-4-ol, bornyl acetate, and linalool.

Verdeguer M., Castañeda L.G., Torres-Pagan N., Llorens-Molina J.A, & Carrubba A. (2020). Control of Erigeron bonariensis with Thymbra capitata, Mentha piperita, Eucalyptus camaldulensis, and Santolina chamaecyparissus Essential Oils. Molecules, 25(3), 562.

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GC-MS and MALDI-TOF Analysis of PEGylated BSA

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An Agilent Technologies (Santa Clara, CA) 6890N/7890 GC system equipped with a split/splitless injector and a flame ionization detector was used. Data acquisition and processing were performed using Agilent ChemStation software.
A Perkin Elmer (Waltham, MA) Clarus 500 GC–MS equipped with a Perkin Elmer TurboMass Quadrupole mass spectrometer was employed for peak identification. Data were acquired in positive ion mode with a scan range of m/z 35–500 at a source temperature of 200°C. The NIST mass‐spectroscopy library was used to identify the peaks present in a test sample. A Waters® (Columbia, MD) Micromass MALDI micro MX, MALDI‐TOF MS was used to confirm PEG 40 000 content in PEGylated BSA.

Wei Z., Bilbulian S., Li J., Pandey R., O'Connor E., Casas‐Finet J, & Cash P.W. (2015). Universal method for the determination of nonionic surfactant content in the presence of protein. Journal of Separation Science, 38(8), 1318-1325.

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GC-MS and GC-FID Analysis of Compounds

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GC-MS analysis was carried out on a Perkin Elmer Clarus 500 GC-MS and gas chromatography-free induction decay (GC-FID) (Waltham, MA, USA) equipped with a Varian VF-5ms (Factor Four of Superchrom) capillary column (60 m × 0.25 mm, film thickness 0.25 µm), according to previous methods [38 (link)] with some modifications. Injector temperature was 250 °C, detector temperature 300 °C, while the column temperature was held at 50 °C and programmed to 170 °C at a rate of 6 °C/min then increased to 270 °C at a rate of 8 °C/min and then kept isothermally at 270 °C for 10 min; helium was used as the carrier gas with 1 mL/min flow; ionization energy was 70 eV and mass ranged from 35 to 450 amu. The components were identified by comparison of their mass spectra to those reported in the Wiley and NIST/NBS (National Institute of Standards and Technology/National Bureau of Standards) libraries. Furthermore, the Linear Retention Indices (LRIs) were calculated and compared with the available retention data reported in the literature. The peak areas of the FID signal were used to calculate the relative percentages of the components without the use of an internal standard and any factor correction. All of the analyses were carried out in triplicate.

Di Sotto A., Gullì M., Minacori M., Mancinelli R., Garzoli S., Percaccio E., Incocciati A., Romaniello D., Mazzanti G., Eufemi M, & Di Giacomo S. (2022). β-Caryophyllene Counteracts Chemoresistance Induced by Cigarette Smoke in Triple-Negative Breast Cancer MDA-MB-468 Cells. Biomedicines, 10(9), 2257.

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Headspace Analysis of Volatile Organic Compounds in Fruit and Leaf Samples

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To investigate the volatile fraction of dried samples, a PerkinElmer Headspace (HS) Turbomatrix 40 autosampler (Waltham, MA, USA) connected to a Clarus 500 GC-MS was used for headspace analysis [45 (link),46 (link)]. To optimize the headspace procedure for the determination of volatile organic compounds (VOCs), parameters such as equilibration time and temperature were adjusted. The sampling procedure was performed as follows: fruit and leaf samples (14 mg and 120 mg, respectively) were put into a 20mL vial with 2 mL of diethyl ether and immediately tightly sealed with crimp aluminum caps and 20-mm white rubber septa (Merck KGaA, Darmstadt, Germany) using a vial crimper. The samples were incubated at 90 °C for 20 min, then the volume of headspace gas was transferred into the capillary column (Rtx-1) by a transfer line. The same temperature program, described in the next paragraph, was used for the GC/MS apparatus.

Carradori S., Cairone F., Garzoli S., Fabrizi G., Iazzetti A., Giusti A.M., Menghini L., Uysal S., Ak G., Zengin G, & Cesa S. (2020). Phytocomplex Characterization and Biological Evaluation of Powdered Fruits and Leaves from Elaeagnus angustifolia. Molecules, 25(9), 2021.

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Volatiles Extraction from Horseradish Pomace

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Volatiles from the horseradish leaves pomace were extracted using SPME. 2.00 ± 0.01 g sample of fresh pomace (Fr) and 1.00 ± 0.01 g of dried pomace were weighed into a 20 mL headspace vial. For SPME extraction, a divinylbenzene/ carboxen/polydimethylsiloxane (DVB/Car/PDMS) fiber (Supelco Inc., Bellefonte, PA, USA) was used. The headspace SPME is based on the absorption of the analytes on a fiber coating placed in the sample's headspace volume and on the partition of the target analytes between the sampling matrix and the fiber. SPME parameters were: incubation time 10 min; extraction temperature 35 ± 1 °C; and extraction duration 10 min. After the extraction, the volatile compounds were thermally desorbed and transferred onto the chromatographic column where they were separated. Desorption parameters were set for 15 min at 250 °C. For the analysis of the SPME extracts, a Perkin Elmer Clarus 500 GC/MS and an Elite-Wax ETR columns (60 m × 0.25 mm i.d.; DF: 0.25 μm) were used. Working conditions were as described by Tomsone et al. [29] (link). All analyses were performed in triplicate.

Tomsone L., Galoburda R., Kruma Z, & Cinkmanis I. (2020). Characterization of dried horseradish leaves pomace: phenolic compounds profile and antioxidant capacity, content of organic acids, pigments and volatile compounds. Eur Food Res Technol., 246(8).

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GC-MS Analysis of Essential Oil Components

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The GC-MS analysis was carried out on Clarus 500 GC-MS (Perkin-Elmer Inc.) apparatus, equipped with the same capillary column and carrier. Its operating conditions are described above for the GC-FID analysis. The ionization source temperature was set at 200°C and the 70 eV electron impact mode was employed. MS spectra were obtained in the full scan mode (mass range m/z 45-500 uma). The total ion chromatograms (TIC) and mass spectra were processed with the Turbomass 5.4 software (Perkin-Elmer Inc.). Retention indices were determined by an injection of the C₈–C₂₅ n-alkanes standard (Supelco, Bellefonte, PE, USA) under the same conditions. The EO components were identified by making a comparison of the calculated retention indices and high probability matches according to a mass spectra computer library search (NIST MS 2.0) and the available data from the literature (Adams, 2007 ). The identification of the following compounds was also confirmed by comparing their experimental linear retention index (LRI) to those of authentic reference standards (Merck KGaA, Darmstadt, Germany): α-pinene, β-pinene, camphene, p-cymene, myrcene, limonene, (Z)-β-ocymene, camphor, terpinolene and terpinen-4-ol.

Roselló J., Llorens-Molina J.A., Larran S., Sempere-Ferre F, & Santamarina M.P. (2024). Biofilm containing the Thymus serpyllum essential oil for rice and cherry tomato conservation. Frontiers in Plant Science, 15, 1362569.

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GC-MS Analysis of Bioactive Compounds

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T h e G C -M S a n a l y s i s o f b i o a c t i ve compounds from plants extract was done using Clarus 500 GC-MS (Perkin Elmer, Shelton, CT, USA). TurboMass version 5. 4.2.1617 was used as a software integrator & controller. An Optima® 1 GC capillary column, Crossbond® 100% dimethyl polysiloxane (30-meter × 0.25 mm ID × 0.25 μm df), Macherey-Nagel, GMBH, Duren, Germany) was used. Al Hashmi et al.,(2013) described a similar setting, but a few modifications were made. In this assay, Helium (purity 99.9999%) was used as a carrier gas, with 0.90 mL/min flow rate. Source (EI+): source was set to 215˚C temperature, GC inlet line was set to 265˚C temperature, with 70 eV Electron energy, and 100V trap-emission. The oven programming went as follows: 50°C temperature initially (with a 5 min hold), then raised to 260˚C (at a rate of 10˚C/min, with 5 min hold), then raised again to 280˚C (at a rate of 10˚C/min, with 2 min hold). Temperature of the injector was set to 265°C, 1.0 μL was injected, and a 50:1 ratio was used for splitting. A total MS scan from 40 to 500 m/z (500 scan/sec) was applied to acquire the sample. The eluted compounds were characterized using NIST 2008, as reported in Mosbah et al.,(2018).

Ali N.M. (2021). Chemical Composition, Antimicrobial Activity and Potential Cytotoxic Effect of Mentha Viridis (Spearmint) Extracts from Saudi Arabia. Oriental Journal Of Chemistry, 37(1), 120-127.

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Volatile Compound Profiling of Fruit Juices

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Volatiles from apple juice and fermented drinks were extracted using solid phase microextraction (SPME). 5ml of juice were weighed in a 20 ml headspace vial and capped with a septum. SPME extraction. A divinylbenzene/carboxen/polydimethylsiloxan e (DVB/Car/PDMS) fiber (Supelco Inc., Bellefonte, PA, USA) was used for headspace SPME sampling. SPME parameters were: incubation time 30 min, extraction temperature 22±2°C, extraction duration 30 min, desorption 15 min, 250°C. For the analysis of the SPME extracts, a Perkin Elmer Clarus 500 GC/MS and a Elite-Wax ETR (60 m x 0.25 mm i.d.; DF 0.25m) was used. Working conditions were: injector 250°C; transfer line to MSD 260°C; oven temperature start 50°C, hold 2 min, programmed from 50 to 100 °C at 5°C min-1 hold 5 min, and from 100 to 210°C at 5°C min-1, hold 15 min; carrier gas (He) 1 ml min-1; split ratio 2:1; ionization EI+; acquisition parameters in full scan mode: scanned m/z 50-300. Compounds were identified by comparison of their mass spectra with mass spectral libraries (Nist98), and by calculation of linear retention indexes and comparison with literature data. All analyses were performed in triplicate. As a quantitative measure, the share in the total GC peak area for each compound is given.

Jyoti S., Sood N., & Singh A. (2021). Process Optimization and Physico-Chemical Evaluation of Apple Ginger Blended Wine along with Different Sweetening Agents. International Journal of Current Microbiology and Applied Sciences, 10(01), 2941-2949.

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Polyaromatic Hydrocarbons and Organochlorine Analysis

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This article is protected by copyright. All rights reserved 100, was used to estimate the amount of TBT being degraded in the environment. Polyaromatic hydrocarbons and organochlorine analysis in sediment and biota including extraction, clean-up, fractionation, and chromatographic analysis were performed as described in Niencheski and Fillmann [19] . PAHs identification and quantification was performed in a gas chromatograph/mass spectrometer (Perkin Elmer ® Clarus 500 -GC-MS) supplied with an Elite-5MS silica capillary column (5% diphenyl-95% dimethylpolysiloxane; 30 m x 0.25 mm x 0.25 µm film thickness).The injector was kept at 280 °C in splitless mode. The oven temperature program started at 40°C, increasing at 10°C min -1 until 60°C and then at 5°C min -1 to 290°C (5 min); finally, at 10°C min -1 until 300°C (10 min). Helium was used as carrier gas (1.5 mL min -1 ).MS operating conditions were: interface 290 °C, ion source 200 °C, and electron energy 70 eV.

Commendatore M.G., Franco M.A., Gomes Costa P., Castro I.B., Fillmann G., Bigatti G., Esteves J.L, & Nievas M.L. (2015). Butyltins, polyaromatic hydrocarbons, organochlorine pesticides, and polychlorinated biphenyls in sediments and bivalve mollusks in a mid-latitude environment from the Patagonian coastal zone. Environmental toxicology and chemistry, 34(12).

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