Nylon net filter

Manufactured by Merck Group

Sourced in United States, Ireland

The Nylon Net Filter is a laboratory filtration device designed to remove particulate matter from liquid samples. It is made of a nylon mesh material that acts as a physical barrier to trap suspended solids while allowing the liquid to pass through. The filter effectively separates and retains particles of various sizes, depending on the specified pore size. This product provides a reliable and consistent method for sample clarification and purification in scientific research and analytical applications.

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Lab products found in correlation

Human anti ec7 mab 3677, by Thermo Fisher Scientific (3 mentions) Anti human igg alexa fluortm 488 pab, by Thermo Fisher Scientific (3 mentions) To protm3 ready flowtm reagent, by Thermo Fisher Scientific (3 mentions) Lsrii flow cytometer, by BD (3 mentions) Sepharose 2b beads, by Merck Group (2 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Syto 9 green fluorescent nucleic acid stain, by Thermo Fisher Scientific (1 mentions) Bz x810, by Keyence (1 mentions) Carboxy h2dcfda, by Thermo Fisher Scientific (1 mentions) Facsaria 3 flow cytometer, by BD (1 mentions) Software version 10, by FlowJo (1 mentions) Tmre staining, by Abcam (1 mentions) Facsaria 3, by BD (1 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Abcam (1 mentions) Ketoconazole, by LGC (1 mentions) Tamoxifen, by LGC (1 mentions) Tolfenamic acid, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Tamoxifen, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions)

32 protocols using nylon net filter

Mitotracker Staining and FACS Analysis

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Fibroblasts seeded in 6-well plates at the density 10⁵ cells per well. On the following day, the media was removed, cells were rinsed with serum-free CMEM and were incubated with 50 nM Mitotracker Red CMXRos (Thermo Fisher) in serum-free CMEM at 37 °C and 5% CO₂ for 30 min.
Following incubation, cells were harvested by Trypsin-EDTA, washed twice with sterile 1× PBS and resuspended in 200 µL 1× PBS complemented with 1.0% (v/v) FBS. Cells were filtered through a 41 µm Nylon Net Filter (Merck Millipore) prior to the FACS measurement.
Data acquisition was performed using a FACS Aria III flow cytometer (BD Biosciences). Data were analyzed with FlowJo software.

Aladdin A., Király R., Boto P., Regdon Z, & Tar K. (2019). Juvenile Huntington’s Disease Skin Fibroblasts Respond with Elevated Parkin Level and Increased Proteasome Activity as a Potential Mechanism to Counterbalance the Pathological Consequences of Mutant Huntingtin Protein. International Journal of Molecular Sciences, 20(21), 5338.

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Water Sample Filtration and Cell Isolation

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Water sample was obtained from an artificial pond at the premises of the National Institute of Advanced Industrial Science and Technology (AIST), Japan, in November 2019. A grab sample was collected in a 2.2-l autoclaved plastic bottle, roughly 5 cm below the surface of the water. To remove large particles and debris, the water sample was passed sequentially through a 41-µm and a 20-µm nylon net filter (Merck Millipore, Billerica, MA, USA). The filtered sample was then centrifuged at 12,000 × g for 20 min at 18 °C and 400 ml of subnatant with cell pellet recovered. The latter was centrifuged (9,510 × g for 20 min at 18 °C) and the recovered cell pellet washed with R2A medium (bacto yeast extract, 0.5 g/l; bacto proteose peptone No. 3, 0.5 g/l; casamino acids, 0.5 g/l; glucose, 0.5 g/l; soluble starch, 0.5 g/l; K₂HPO₄, 0.3 g/l; MgSO₄⋅7H₂O, 0.05 g/l; sodium pyruvate, 0.3 g/l). The washing step was repeated two times and cells were finally resuspended in 17 ml of R2A medium. Cells were stained with SYTO 9 Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific, Waltham, MA, USA) and counted by fluorescence microscopy.

Saito K., Ota Y., Tourlousse D.M., Matsukura S., Fujitani H., Morita M., Tsuneda S, & Noda N. (2021). Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort. Scientific Reports, 11, 9506.

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Induction of Chlamydospore Invasion by RSSC

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GFP-expressing RSSC strains were inoculated into 2× BG medium (2 mL) containing kanamycin (50 μg/mL) and cultured overnight by shaking. A nylon net filter (11 μm, 47 mm, Merck) was placed on the surface of a BG agar medium (25 mL in 90-mm plastic dish) containing kanamycin (100 μg/mL). After the B medium (50 μL) was added to the top of the net, another nylon net (20 μm, 47 mm, Merck) was placed on top. RSSC cell suspension (5 μL) and F. oxysporum NBRC 31213 spore suspension (5 μL) were spotted on the top net, 15 mm apart from each other. The plate was incubated at 30°C for 3 days. The top net filter was removed from the medium and washed thoroughly with MilliQ water to remove the bacteria cells. Chlamydospores were collected by rubbing the surface using a cover glass. The collected chlamydospores were washed with MilliQ water (0.5 mL) and observed under a fluorescence microscope (BZ-X810, Keyence). The invasion rate (% = chlamydospores with RSSC/total chlamydospores × 100) per condition was calculated from three to five dishes.

Tsumori C., Matsuo S., Murai Y, & Kai K. (2023). Quorum Sensing-Dependent Invasion of Ralstonia solanacearum into Fusarium oxysporum Chlamydospores. Microbiology Spectrum, 11(4), e00036-23.

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Measuring Intracellular ROS Using DCFDA

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Fibroblasts were seeded in 6-well plates at the density 10⁵ cells per well. On the following day, cells were incubated with 1 μM Carboxy-H2DCFDA (Thermo Fisher) in CMEM with reduced FBS (2%) for 30 min at 5% CO₂ and 37 °C. Cells were harvested by Trypsin-EDTA, rinsed with 1× PBS and resuspended in 1× PBS complemented with 1% (v/v) FBS. 6-carboxy-2’,7’-dichlorodihydrofluorescein diacetate is a chemically reduced, acetylated form of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. This nonfluorescent molecule is readily converted to a green-fluorescent form when the acetate groups are removed by intracellular esterases and oxidation (by the activity of ROS) occurs within the cell.
Cells were filtered through a 41 µm Nylon Net Filter (Merck Millipore, MilliporeSigma, Burlington, MA, USA prior to the FACS measurement. Data acquisition was performed using a FACS Aria III flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). Data were analyzed with FlowJo software version 10 (FlowJo LLC, Ashland, OR, USA).

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Mitochondrial Membrane Potential Assay

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Cells were treated with and without 20 µM of Carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone (FCCP) (Abcam, Cambridge, UK) for 10 min prior the 100 nM TMRE staining (Abcam) in CMEM at 5% CO₂ and 37 °C for 10 min. Following incubation cells were harvested by Trypsin-EDTA, washed twice with sterile 1× PBS and resuspended in 200 µL 1× PBS complemented with 1.0% (v/v) FBS. Cells were filtered through a 41 µm Nylon Net Filter (Merck Millipore) prior to the FACS measurement. Data acquisition was performed using a FACS Aria III flow cytometer (BD Biosciences). Data were analyzed with FlowJo software.

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Characterization of MMC Drug Carrier

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Five PWSDs were selected and MMC was synthesized in-house. MMC has been extensively characterized as a drug carrier in previous work and had a mean pore size of 5 nm, a specific surface area of 650 m 2 /g, and a total pore volume of 0.81 cm 3 /g (11, 18) . Hydroxypropyl methyl cellulose (HPMC) (Methocel E4M Premium CR) was generously donated by Colorcon (Dartford, UK). Fenofibrate, tolfenamic acid, tamoxifen, hydrocortisone, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St Louis, MO, US). tamoxifen and ketoconazole were purchased from Toronto Research Chemicals (Toronto, CAN). All drugs were used as received. The nylon net filter, 11-μm pore size, used in the protective filters were purchased from Merck Millipore (Billerica, MA, US). FaSSGF/FaSSIF/FeSSIF powder was purchased from biorelevant.com (Croydon, UK).

Alvebratt C., Cheung O., Strømme M, & Bergström C.A.S. (2018). A Modified In Situ Method to Determine Release from a Complex Drug Carrier in Particle-Rich Suspensions. AAPS PharmSciTech, 19(7).

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Cannabis Trichome Isolation in Flowering

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Cannabis trichomes in this research were isolated according to Yerger et al. [28] with slight modifications. Fresh flowers of C. sativa, variety Bediol, were put into liquid nitrogen. Floral leaves and the stigma were removed using forceps, with occasional resubmerging of the flowers in liquid nitrogen. Afterwards, a 5-to 10-g flower sample was transferred into a 50-mL falcon tube and placed in liquid nitrogen. The tube was then removed from the liquid nitrogen tank and approximately 2 to 3 cm 3 of finely powdered dry ice (prepared by wrapping a piece in clean paper towels and crushing with a pestle) were added. Immediately, the tube was loosely capped and vortexed at maximum speed for approximately 1 min, and then the flowers were removed. To obtain the trichomes, the content of each tube was sieved through a nylon net filter with a pore diameter of 140 µm (Merck Millipore) into a 500-mL glass beaker surrounded by dry ice. The trichomes were subsequently transferred into 2 mL frozen microcentrifuge tubes with a spatula; the samples were placed in liquid nitrogen.
In this work, trichomes were isolated at 4 different harvest times during the flowering period, namely, weeks 5, 6, 7, and 8. Six biological replicates of the Cannabis trichomes were collected at each harvest, thus, in total, twenty-four samples were prepared.

Happyana N, & Kayser O. (2016). Monitoring Metabolite Profiles of Cannabis sativa L. Trichomes during Flowering Period Using 1H NMR-Based Metabolomics and Real-Time PCR. Planta medica, 82(13).

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Fluorescent Fibrin Emboli Generation

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Fibrin emboli were prepared ex vivo using a previously reported method with modification. 15 (link) Briefly, 10 mg of human fibrinogen (Sigma-Aldrich Co.; St Louis, MO, USA), 0.2 units of human thrombin (Sigma-Aldrich Co.), and 6 mg of rhodamine B isothiocyanate-dextran 70 (RITC-dextran; 8 mg/ml; Sigma-Aldrich Co.) were mixed with 1 mL of normal saline, and the mixture was refrigerated at 4°C overnight. Before injection of the embolic mixture on the following day, the fibrin emboli were homogenized using 0.5 mL normal saline and then passed through a hydrophilic nylon net filter (pore size, 41.0 μm; Merck Millipore Ltd., Burlington, MA, USA). The fibrin emboli could be visualized under a fluorescence microscope (Fig. 2).

Katsumata M., Oki K., Tsukada N., Abe T., Itoh Y., Takahashi S, & Suzuki N. (2019). Rivaroxaban Promotes Reduction of Embolus Size within Cerebrocortical Microvessels in a Mouse Model of Embolic Stroke. The Keio journal of medicine, 68(3).

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Preparation and Characterization of KIOM-MA and KIOM-MA128

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KIOM-MA and KIOM-MA128 were prepared according to previously described methods [6 (link)]; the herbal medicines in KIOM-MA (Glycyrrhizae radix, Polygoni cuspidati Rhizoma, Sophorae radix, Cnidii rhizoma, Arctii fructus, etc. [6 (link),14 ], was obtained from the Yeongcheon Oriental Herbal Market (Yeongcheon, Korea), and then identified by Dr. Ki-Hwan Bae, Professor Emeritus at the College of Pharmacy, Chungnam National University (Daejeon, Korea). KIOM-MA (1 kg) was boiled in distilled water (10 liters) for approximately 3 h at 115 °C. The aqueous extract was filtered through a testing sieve (Aperture 500 μm and 150 μm). The filtered extract was inoculated with Lactobacillus rhamosus (1 × 10⁵–10⁷ CFU/mL; KFRI 128, KCTC 2182) provided by the Korea Food Research Institute, the pH of which was adjusted to 7.0 with 1 N NaOH and then autoclaved for 5 min, and then incubated for 48 h at 37 °C. KIOM-MA128 was filtered through a nylon net filter (60 μm; Millipore Co., Denver, MA, USA), and then deposited overnight. The supernatant was lyophilized, and then the dried pellet (the yield, 20.44%) was stored at −20°C until use. KIOM-MA128 were dissolved in a 10% DMSO solution or deionized water for in vitro or in vivo studies, respectively.

Park K.I., Kim D.G., Yoo J.M, & Ma J.Y. (2016). The Herbal Medicine KIOM-MA128 Inhibits the Antigen/IgE-Mediated Allergic Response in Vitro and in Vivo. Molecules, 21(8), 1015.

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Extraction of Colla corii asini

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Colla corii asini were purchased from the Korea Medicinal Herbs Association (Yeongcheon, Korea). The identification of Colla corii asini was confirmed by Professor KiHwan Bae of the College of Pharmacy, Chungnam National University (Daejeon, Korea), and all voucher specimens were deposited in the herbal bank in the Korea Institute of Oriental Medicine (KIOM, Korea).
To prepare the Colla corii asini ethanol extract (CEE), Colla corii asini (50 g) was grounded to powder, then suspended in 70% ethanol (300 ml) on shaking incubator (100 rpm) for 24 h at 40 °C. The solution was filtered through a nylon net filter (60 μm; Millipore Co., Denver, MA, USA), and then deposited overnight. The supernatant was lyophilized, and then the dried pellet (the yield, 1.66%) was stored at −20 °C until use. Also, to prepared the Colla corii asini water extract (CWE), Colla corii asini (50 g) were placed in 1000 mL distilled water and then extracted during 2 h of heating at 115 °C (Gyeongseo Extractor Cosmos-600, Inchon, Korea), and the solution was filtered using standard testing sieves (140 μm) (Retsch, Haan, Germany). The supernatant was lyophilized, and then the dried pellet (the yield, 38.70%) was stored at −20 °C until use.

Park K.I., Lee M.R., Oh T.W., Kim K.Y, & Ma J.Y. (2017). Antibacterial activity and effects of Colla corii asini on Salmonella typhimurium invasion in vitro and in vivo. BMC Complementary and Alternative Medicine, 17, 520.

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