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2 protocols using tetrabutylammonium hydroxide solution tbaoh

1

Pluronic F-127 Hydrogel Synthesis

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Pluronic F-127 (Sigma-Aldrich), anhydrous toluene (Wako, 99.5%), trimethylamine (Wako, 99.0%), methacryloyl chloride (TCI, 90%), diethyl ether (Wako, 99.5%), montmorillonite (The Clay Science Society of Japan), pentaethylene glycol monodecyl ether (C10E5) (Sigma Aldrich, 97.0%), potassium carbonate (K2CO3) (Wako, 98.5%), niobium(v) oxide (Nb2O5) (Wako, 99.9%), hydrochloric acid (HCl) (Wako, 35.0%), tetrabutylammonium hydroxide solution (TBAOH) (Sigma-Aldrich, 40 wt%), and 2-hydroxymethyl propiophenone (TCI, 96.0%) were used in this study.
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2

Synthesis and Characterization of Cisplatin-Hyaluronate Conjugates

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Cis-Dichlorodiamine platinum (II) (cisplatin) was purchased from Strem Chemicals, Inc. (Newburyport, MA). Sodium hyaluronate was purchased from Contipro Inc (Dolní Dobrouč, Czech Republic). 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU), and 3-(3-Dimethylaminopropyl)-1-ethyl-carbodiimide hydrochloride (EDC-HCl) was purchased from Chem-Impex International, Inc. (Wood Dale, IL). 1-Hydroxybenzotriazole hydrate (HOBt•H2O) was purchased from Advanced ChemTech (Louisville, KY). N-(tert-Butoxycarbonyl)glycine (Boc-Gly-OH), hydrogen peroxide solution (H2O2, 30 wt.% in H2O), succinic anhydride, (±)-α-tocopherol, N,N′-Dicyclohexylcarbodiimide (DCC), N,N-Dimethylpyridin-4-amine (DMAP), trifluoroacetic acid (TFA), N,N-Diisopropylethylamine (DIPEA), oxaliplatin, tetrabutylammonium hydroxide solution (TBA-OH, 40 wt.% in H2O), and anhydrous organic solvents were purchased from Sigma-Aldrich (Saint Louis, MO). Alexa Fluor ® 488 anti-CD8a, Alexa Fluor® 594 anti-CD11b, and Alexa Fluor® 647 anti-CD11c antibodies were purchased from BioLegend (San Diego, CA). All other chemicals and organic solvents were purchased from Thermo Fisher Scientific (Waltham, MA).
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