Beh c18 column 1000 mm 2.1 mm 1 7 m

The furans were quantified in a UPLC-MS (XEVO TQS Micro) (Waters, Milford, CT, USA) integrated by an ACQUITY Class I Waters (Milford, CT, USA) binary pumping system. The system was equipped with a waters brand BEH C₁₈ column 1000 mm × 2.1 mm × 1.7 µm (Waters, Dublin, Ireland) and probe type APcI (Table 4). The temperature was 150 °C, the probe temperature was 450 °C, the cone gas flow was 100 L/h, and the desolvation gas flow was 600 L/h. The acquisition was performed in multiple reaction monitoring (MRM) modes. The mobile phases: (A) water: formic acid (99.7:0.3 v/v) and (B) methanol. The elution gradient was as follows: 70% A as an initial condition, 70% A at 1.5 min, 50% A at 2 min, 0.1% A at 2.01 min, 0.1% A at 3 min, 70% A at 3.01 min and 70% A at 5 min. The quantification of the compounds was carried out by preparing calibration curves with the standards 1, 2.5, 5, 12.5, 25, and 50 mg/L for furans.

The phenol compounds were quantified in a UPLC-MS (XEVO TQS Micro) (Waters, Milford, CT, USA) integrated by an ACQUITY Class I Waters (Milford, CT, USA) binary pumping system. The system was equipped with a waters brand BEH C₁₈ column 1000 mm × 2.1 mm × 1.7 µm (Waters, Dublin, Ireland) and probe ESI (Table 4). The source temperature was 130 °C, the probe temperature was 450 °C, the cone gas flow was 20 L/h, and the desolvation gas was 800 L/h. The acquisition was performed in multiple reaction monitoring (MRM) modes. The gradient with mobile phases: (A) water: formic acid (99.7:0.3 v/v) and (B) methanol. The elution gradient was as follows: 90% A as the initial condition, 30% A at 11 min, and 90% A at 15 min. The quantification of the compounds was carried out by preparing calibration curves with the standards 0.1, 0.5, 1, 2.5, and 5 mg/L for phenolic compounds.