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W6 32

Manufactured by BD
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Sourced in Germany

The W6/32 is a laboratory equipment product designed for general laboratory use. It serves as a versatile tool to support various laboratory operations and procedures. The core function of the W6/32 is to provide a reliable and consistent platform for laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

G155 178, by BD (1 mentions) Mopc 21, by BD (1 mentions) Anti mouse af647, by Thermo Fisher Scientific (1 mentions) Accuri c6 cytometer, by BD (1 mentions)

2 protocols using w6 32

1

Cell Surface Antigen Analysis by FACS

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Cell surface antigens were analysed by FACS on a BD FACSCalibur after 20 min staining at 4°C with antibodies to HLA class I–FITC (W6/32; BD), HLA-DR–FITC (L243; BD), IgG1–APC (MOPC-21; BD), IgG2a–FITC (G155-178; BD), IgG2a–Phycoerythrin (G155-178; BD), anti-CD46–APC (MEM-258; ImmunoTools, Friesoythe, Germany), anti-CD55–Phycoerythrin (IA10; BD) and anti-CD59–APC (OV9A2, eBioscience).
van der Torren C.R., Zaldumbide A., Duinkerken G., Brand-Schaaf S.H., Peakman M., Stangé G., Martinson L., Kroon E., Brandon E.P., Pipeleers D, & Roep B.O. (2016). Immunogenicity of human embryonic stem cell-derived beta cells. Diabetologia, 60(1), 126-133.
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2

Flow Cytometry Analysis of Cell Markers

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Cells were dissociated with TrypLE, stained with antibodies targeting HLA (W632), PDGFRα (BD Pharmingen) or CD155 (D171; Invitrogen) followed by anti-mouse AF647 (Thermo), before being washed and fixed in 4% PFA, then run on a Accuri C6 cytometer (BD) and analysed in FlowJo. 'All gates were set using a non-binding isotype matched control antibody (for the negative gate), and cells that had been transduced with a control RAd vector lacking expression of Cas9 (for the positive gate).
Statkute E., Wang E.C.Y, & Stanton R.J. (2022). An Optimized CRISPR/Cas9 Adenovirus Vector (AdZ-CRISPR) for High-Throughput Cloning of sgRNA, Using Enhanced sgRNA and Cas9 Variants. Human gene therapy, 33(17-18).
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