Beta cell calcium dynamics were imaged using an upright confocal microscope system Leica TCS SP5 AOBS Tandem II with a 20X HCX APO L water immersion objective, NA 1.0, and an inverted confocal system Leica TCS SP5 DMI6000 CS with a 20X HC PL APO water/oil immersion objective, NA 0.7 (all from Leica Microsystems, Germany). A 488 nm argon laser was used to excite the fluorescent dye, and a Leica HyD hybrid detector operating in the 500–700 nm range was used to detect the fluorescence that was released (all from Leica Microsystems, Germany), as previously described [27 (link),122 (link)]. The resolution used for time series acquisition was 512 X 512 pixels with a frequency of 2–10 Hz.
Tcs sp5 aobs tandem 2
Manufactured by Leica
Sourced in Germany
The Leica TCS SP5 AOBS Tandem II is a high-performance confocal laser scanning microscope system designed for advanced scientific research. It features a tandem scanning system with an Acousto-Optical Beam Splitter (AOBS) for efficient and flexible laser control. The system is capable of multicolor imaging and can be configured with a range of laser lines to suit various applications.
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2 protocols using tcs sp5 aobs tandem 2
1
Imaging Beta Cell Calcium Dynamics
2
Confocal Calcium Imaging Technique
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[Ca2+]i imaging was performed using an upright confocal microscope system Leica TCS SP5 AOBS Tandem II with a 20X HCX APO L water immersion objective, NA 1.0, and an inverted confocal system Leica TCS SP5 DMI6000 CS with a 20X HC PL APO water/oil immersion objective, NA 0.7 (all from Leica Microsystems, Germany). The time series were acquired at a resolution of 512 X 512 pixels with a frequency of 2 Hz. The calcium reporter dye was excited with a 488 nm argon laser line, and the emitted fluorescence was detected with a Leica HyD hybrid detector in the 500-700 nm range (all from Leica Microsystems, Germany), as previously described (51 (link), 54 (link), 55 (link)).
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