Architect 8100 chemistry analyzer

Blood samples were obtained from the facial vein of mice, collected in tubes containing K₂-EDTA (Wiener lab), and after centrifugation at 2000 × g for 10 min at room temperature, plasma samples were separated. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), creatine kinase (CK), and lactate dehydrogenase (LDH) were measured by UV- or -kinetics method at different days post-secondary infection with DENV-2 (0, 4, and 7 days) using an Architect 8100 chemistry analyzer (Abbott Laboratories). Platelet counts were measured by microscopy on a CELLDYN 3700 (Abbott Laboratories) according to the manufacturer's instructions.
Increase of liver enzymes was defined as ALT and/or AST plasma values significantly above the ones obtained for control mice. Platelet counts significantly lower than the value obtained for control mice were also considered a primary outcome of disease. Plasma values of CK, LDH and ALP significantly higher than the ones obtained for control mice were considered secondary outcomes of disease. All normal laboratory values for C57BL/6 mice were obtained from the Comparative Pathology Laboratory, Vanderbilt University Medical Center, and the Mouse Phenome Database at The Jackson Laboratory.

Suckling mice were euthanized at 8 days PI and blood samples were collected in tubes containing K₂‐EDTA (Wiener lab). After centrifugation at 2000× g for 10 minutes at room temperature, plasma samples were separated. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), and lactate dehydrogenase (LDH) were measured by UV kinetics‐, kinetics‐ or enzymatic‐ method at 8 days PI using an Architect 8100 chemistry analyzer (Abbott Laboratories). Platelet counts were measured by microscopy on a CELLDYN 3700 (Abbott Laboratories) according to the manufacturer´s instructions.
Transaminitis was defined as ALT and/or AST plasma values significantly above the ones obtained for control mice. Plasma values of LDH and CK significantly higher than the values obtained for control mice were considered outcomes of disease. Platelet count significantly lower than the value obtained for control mice was also considered an outcome of disease.