Horseradish peroxidase hrp conjugated goat anti mouse or anti rabbit igg

An anti-KSHV LANA rat monoclonal antibody (MAb) was purchased from Advanced Biotechnologies Inc. (Columbia, MD, USA)^{89 (link)}. The anti-phospho-c-Met (Y1356) rabbit polyclonal antibody (PAb) was obtained from Abcam (Cambridge, MA, USA). The anti-c-Met rabbit MAb and anti-Flag M2 rabbit MAb were obtained from Cell Signaling Technologies (Beverly, MA, USA). The anti-CD82 rabbit PAb, anti-b-FGF goat PAb, anti-GAPDH mouse MAb, anti-α-Tubulin mouse MAb, and horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit IgG were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-His mouse MAb, anti-GFP rabbit antibody, and anti-mouse immunoglobulin G (IgG) were from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China). Western-blotting analysis was performed as previously described^{90 (link), 91 (link)}. Transfection of HUVECs was performed with the Effectence transfection reagent (Qiagen, Valencia, CA, USA) while transfections in other cells were performed with Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. PF-2341066, a c-Met inhibitor, was purchased from Selleck Chemicals (Shanghai, China).