N-acetyl-L-cysteine (NAC; A9165), MG132 (474790), lactic acid (252476), actinomycin D (A9415), and sodium oxamate (O2751) were purchased from Sigma-Aldrich (USA). The MAPK inhibitors PD98059 (S1177), SB203580 (S1076), and SP600125 (S1460) were purchased from Selleck Chemicals (USA). The fluorogenic probe, 2′, 7′-dichlorodihydrofluorescein diacetate (H2DCFDA; D399) was obtained from Thermo Fisher Scientific (USA). Primary antibodies against p-ERK1/2-Thr202/Tyr204 (#4370), ERK1/2 (#9102), β-actin (#3700), and LDHA (#2012) were purchased from Cell Signaling Technology (USA). Antibodies against HIF-1α (MAB1536), HIF-2α (NB100-122), MKP-3 (ab76310), and ubiquitin (ab134953) were obtained from R&D Systems (USA), Novus Biologicals (USA), and Abcam (UK), respectively. Secondary antibodies, including anti-mouse HRP-linked IgG antibody (sc-516102) and anti-rabbit HRP-linked IgG antibody (sc-2357), were obtained from Santa Cruz Biotechnology (USA).
Ab76310
Manufactured by R&D Systems
Sourced in United States
The ab76310 is a laboratory instrument designed for general scientific research applications. It is a high-performance device capable of performing various analytical and experimental tasks. The core function of this equipment is to facilitate data collection and analysis, enabling researchers to conduct their investigations efficiently.
Automatically generated - may contain errors
Lab products found in correlation
Sodium oxamate, by Merck Group (1 mentions)
Sp600125 s1460, by Selleck Chemicals (1 mentions)
Mab1536, by R&D Systems (1 mentions)
Nb100 122, by R&D Systems (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Integrin β1, by Abcam (1 mentions)
Alexa 594, by Thermo Fisher Scientific (1 mentions)
Ab735, by Abcam (1 mentions)
Hpa039335, by Merck Group (1 mentions)
2 protocols using ab76310
1
Hypoxia-Induced Signaling Pathway Modulation
2
Comprehensive Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies against the following proteins were used: P-ERK (Cell Signaling # 9101; western blot – 1:1000 dilution), ERK (Cell Signaling # 9102; western blot – 1:1000), P-p38 (Cell Signaling # 9211; western blot – 1:1000), p38 (Cell Signaling # 9212; western blot – 1:1000), Cyclophilin B (R and D # MAB5410; western blot – 1:4000), MKP-3/DUSP6 (Abcam # ab76310; western blot - 1:1000 and R and D Systems # MAB3576-SP; immunostaining – 1:200), PPTC7 (Abcam # ab122548; western-blot 1:250 and Sigma # HPA039335; immunostaining – 1:200), PTPN1/PTP1B (Sigma # HPA012542; western-blot - 1:500; R and D Systems # AF1366-SP; immunostaining – 1:200), PTPN13 (R and D Systems # AF3577; western-blot - 1:300 and immunostaining – 1:200), PPP3CA/Calcineurin A (Sigma # HPA012778; western-blot 1/1000 and R and D Systems # MAB2839-SP; immunostaining – 1:200), DUSP10 (Abcam # 140123; western-blot - 1:1000 and immunostaining – 1:200), TP63 (SCBT # sc-8431 or Abcam # ab735; immunostaining – 1:100), Involucrin (SY5, in-house; immunostaining – 1:500, or DH1, in-house; immunostaining – 1:200), Ki67 (Abcam # ab16667; immunostaining – 1:100) and β1-Integrin (clone P5D2, in-house; immunostaining – 1:200). Species-specific secondary antibodies conjugated to Alexa 488 or Alexa 594 were purchased from Molecular Probes, and HRP-conjugated second antibodies were purchased from Amersham and Jackson ImmunoResearch.
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