Catalase activity assay kit ab83464

Spin cups were purchased from ThermoFisher Scientific, and were supplied with 10 µm filters attached; hydrophilic 8 µm membrane filters were purchased from Merck Millipore and used as supplied. Protease inhibitor cocktail ab201111 was purchased from Abcam. Sephadex G-50 was purchased from Sigma Aldrich, and equilibrated in phosphate-buffered saline (PBS). The 0.22 µm filters were purchased from Merck Millipore.
Lipopolysaccharide (LPS) from Escherichia coli O111:B4 was purchased from Sigma Aldrich. Measure-iT^TM High-Sensitivity Nitrite Assay Kit (M36051) was purchased from ThermoFisher Scientific. Biolegend Mouse ELISA Max^TM Deluxe (TNF-α, IL-1β, IL-6 and IL-10) were purchased from BioLegend. Total Antioxidant Capacity (TAC) assay kit (ab65329), Catalase Activity assay kit (ab83464) and GST assay kit (ab65326) were purchased from Abcam.

Estimation of pancreatic tissue content of malondialdehyde (MDA) was done by using the thiobarbituric acid reaction explained by Draper et al., and the result was stated in nmol/g tissue [20 (link)]. Total antioxidants were analyzed according to the method described by Koracevic et al. via the kit (Cat. No. # TA 25 12) delivered from Bio-Diagnostics, Dokki, Giza, Egypt. The result was presented as mmol/L [21 (link)].
Catalase activity (Catalase Activity Assay Kit-ab83464, Abcam, Hong Kong, China) reduced glutathione (Reduced Glutathione (GSH), Endogenous antioxidant ab142044, Abcam) and Superoxide Dismutase (SOD) (Superoxide Dismutase Activity Assay Colorimetric Kit-ab65354, Abcam) were determined according to the manufacturer’s instruction in serum samples [22 ,23 ,24 (link)]. The parameters were represented as U/mL, μmol/mL, and U/mL, respectively.