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Orion perphect ross

Manufactured by Thermo Fisher Scientific
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The Orion PerpHecT ROSS is a pH electrode designed for accurate and reliable pH measurement in a variety of applications. It features a ROSS-type electrode with a double-junction reference system for improved performance and reduced fouling. The electrode is built to withstand harsh environments and provide stable, repeatable pH readings.

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Lab products found in correlation

Amicon ultra, by Merck Group (1 mentions) 96 well pcr plate, by Avantor (1 mentions)

5 protocols using orion perphect ross

1

Analyzing Candida albicans Secretome

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To analyze the role of soluble factors released or depleted by C. albicans during infection, Candida-conditioned medium (CCM) was prepared. For this, C. albicans cultures were prepared as described above. Then, 106 C. albicans cells/ml were inoculated into KBM in suitable multiwell plates and incubated under standard cell culture conditions for 24 h. The supernatant was harvested and sterile-filtered using a pore size of 0.2 µm. Aliquots were frozen at -20°C and thawed on demand in a water bath at 37°C prior to experiments. For size exclusion treatment, aliquots were thawed on ice and CCM was separated through centrifugal filters with molecular weight cut offs between 3 and 100 kDa (Amicon®Ultra, Millipore). For heat-inactivation CCM was incubated for 30 min at 72°C before the experiment. KBM treated in the same way was used as control. The pH of CCM and KBM was measured using a pH electrode (Orion™ PerpHecT™ ROSS™; Thermo Fisher Scientific) after equilibration to cell culture conditions for 5 h.
Niemiec M.J., Kapitan M., Himmel M., Döll K., Krüger T., Köllner T.G., Auge I., Kage F., Alteri C.J., Mobley H.L., Monsen T., Linde S., Nietzsche S., Kniemeyer O., Brakhage A.A, & Jacobsen I.D. (2022). Augmented Enterocyte Damage During Candida albicans and Proteus mirabilis Coinfection. Frontiers in Cellular and Infection Microbiology, 12, 866416.
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2

Determining pKa via NMR Spectroscopy

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NMR samples for pKa determination contained initially 9 × 10–3 mol/L PTD-OMe in an aqueous formic acid/formate buffer containing
10 vol % of D2O. pH was measured with a microelectrode
(Orion PerpHecT ROSS, Thermo Fisher Scientific) and a pH meter (Orion
Star, Thermo Fisher Scientific) before and after NMR measurement.
The pH was adjusted with 1, 0.1, or 0.01 mol/L HCl or NaOH solutions.
Weßling P., Trumm M., Macerata E., Ossola A., Mossini E., Gullo M.C., Arduini A., Casnati A., Mariani M., Adam C., Geist A, & Panak P.J. (2019). Activation of the Aromatic Core of 3,3′-(Pyridine-2,6-diylbis(1H-1,2,3-triazole-4,1-diyl))bis(propan-1-ol)—Effects on Extraction Performance, Stability Constants, and Basicity. Inorganic Chemistry, 58(21), 14642-14651.
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3

Bacterial Supernatant pH Measurement

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pH measurements of bacterial supernatants were performed using a refillable, glass double junction electrode (Orion™ PerpHecT™ ROSS™, Thermo Scientific).
Weiss A.S., Burrichter A.G., Durai Raj A.C., von Strempel A., Meng C., Kleigrewe K., Münch P.C., Rössler L., Huber C., Eisenreich W., Jochum L.M., Göing S., Jung K., Lincetto C., Hübner J., Marinos G., Zimmermann J., Kaleta C., Sanchez A, & Stecher B. (2021). In vitro interaction network of a synthetic gut bacterial community. The ISME Journal, 16(4), 1095-1109.
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4

Bacterial Supernatant pH Measurement

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pH measurements of bacterial supernatants were performed using a refillable, glass double junction electrode (Orion™ PerpHecT™ ROSS™, Thermo Scientific).
Weiss A.S., Niedermeier L.S., von Strempel A., Burrichter A.G., Ring D., Meng C., Kleigrewe K., Lincetto C., Hübner J, & Stecher B. (2023). Nutritional and host environments determine community ecology and keystone species in a synthetic gut bacterial community. Nature Communications, 14, 4780.
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5

Microbial Culture pH Measurement

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To measure the pH of the microbial cultures, 170µl of sample were transferred into 96-well PCR plates (VWR, Radnor, USA) and the pH was measured with a pH microelectrode (Orion PerpHecT ROSS, Thermo Fisher Scientific, Waltham, USA ).
Ratzke C., Barrere J, & Gore J. (2020). Strength of species interactions determines biodiversity and stability in microbial communities. Nature ecology & evolution, 4(3).
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