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Mouse oct4

Manufactured by BD

Mouse Oct4 is a laboratory reagent used in cell biology research. It is a recombinant protein that represents the transcription factor Oct4, which is known to play a critical role in the maintenance of stem cell pluripotency. This product can be used for various research applications, such as protein expression, purification, and functional studies.

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3 protocols using mouse oct4

1

Antibody Characterization for Stem Cell Research

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The following antibodies were used: rabbit Flag (Sigma-Aldrich), 1:300 for immunostaining, 1:1,000 for Western blot; mouse HA (12CA5; Roche), 1:200; rabbit HA (Abcam), 1:200; rabbit H1.10 (Abcam), 1:100; mouse H1.0 (Santa Cruz), 1:200; mouse H3K9me3 (Active Motif), 1:100; rabbit H3K9ac (Cell Signaling Technologies), 1:100; mouse OCT4 (BD Biosciences), 1:200; goat OCT4 (N19; Santa Cruz), 1:200; mouse SSEA-1 (BD Biosciences); rabbit Lamin-B1 (Abcam), 1:100; and mouse H1 (AE4; Abcam), 1:100. For details about the antibody applicability and conditions, see Table 1.
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2

Antibody Panel for Stem Cell Analysis

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Antibodies used in this work were the following: mouse turboGFP (OTI2H8, Origene), rabbit turboGFP (PA5-22688, ThermoFisher), rabbit Zscan4 (AB4340, EMD Millipore), rabbit MuERVL-Gag (R1501-2, Hangzhou HuaAn Biotechnology), chicken eGFP (ab13970, Abcam), rabbit H2AK119ub (8240, Cell Signaling), goat Rex1 (sc-50670, Santa Cruz), goat Oct4 (sc-8628, Santa Cruz), mouse Oct4 (611203, BDBiosciences), goat Sox2 (sc-17320, Santa Cruz), rabbit Prdm14 (gift from D. Reinberg, 62 , rabbit Tfap2c (sc-8977, Santa Cruz).
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3

Antibody Panel for Stem Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this work were the following: mouse turboGFP (OTI2H8, Origene), rabbit turboGFP (PA5-22688, ThermoFisher), rabbit Zscan4 (AB4340, EMD Millipore), rabbit MuERVL-Gag (R1501-2, Hangzhou HuaAn Biotechnology), chicken eGFP (ab13970, Abcam), rabbit H2AK119ub (8240, Cell Signaling), goat Rex1 (sc-50670, Santa Cruz), goat Oct4 (sc-8628, Santa Cruz), mouse Oct4 (611203, BDBiosciences), goat Sox2 (sc-17320, Santa Cruz), rabbit Prdm14 (gift from D. Reinberg, 62 , rabbit Tfap2c (sc-8977, Santa Cruz).
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