Losartan used in our study was a gift from Xiuzheng Pharmaceutical Group Company Limited. Edathamil disodium (EDTA) was acquired from Sigma-Aldrich (Shanghai, China), whereas Dulbecco’s modification of Eagle's medium, Serum, total RNA extraction reagent, and Trypsin were procured from GIBCO Life Technology Company (Shanghai, China). Rabbit polyclonal antibodies against p65 were obtained from Santa Cruz Biotechnology (Dallas, Texas). Yahui Biomedical Engineering Company (Beijing, China) was the source for α-32P-deoxycytidine triphosphate.
Rabbit polyclonal antibodies against p65
Manufactured by Santa Cruz Biotechnology
Rabbit polyclonal antibodies against p65 are a laboratory tool used to detect and study the p65 subunit of the NF-kB transcription factor in biological samples. These antibodies are produced by immunizing rabbits with a p65-specific antigen, resulting in a polyclonal population of antibodies that recognize multiple epitopes on the p65 protein.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modification of eagle s medium, by Thermo Fisher Scientific (1 mentions)
Uplansapo 20x 0.75 objective, by Olympus (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Ix71 microscope, by Olympus (1 mentions)
2 protocols using rabbit polyclonal antibodies against p65
1
Losartan Treatment Effects Protocol
2
Immunostaining of IL-1β-Stimulated HUVECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Post-confluent HUVECs cultured on fibronectin-coated glass coverslips were treated with comfrey or TAK inhibitor for 30 min before IL-1β stimulation. At the indicated time points, cells were fixed for 15 min with 4% paraformaldehyde (Sigma), permeable for 30 min with 0.1% Triton X-100 (Sigma), washed with PBS, and finally blocked for 1 h with 3% BSA-TBS-T. For immunostaining, rabbit polyclonal antibodies against p65 (Santa Cruz) (1:500) were used with a secondary antibody conjugated to an Alexa-Fluor 488 goat antibody against rabbit IgG antibody (Invitrogen) in a ratio of 1:1000. Cells were contrasted for 15 min with Alexa Fluor 568 phalloidin (1:1000) and 5 min with 4',6-diamidino-2phenylindole (DAPI, Life Technologies; 1:10,000). The samples were examined using an Olympus IX71 microscope with a UPlanSApo 20x/0.75 objective. The images were processed using the ImageJ program.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!