Anti cd25 pc61.5

Manufactured by BioLegend

Anti-CD25 (PC61.5) is a lab equipment product that binds to the CD25 antigen, which is a component of the interleukin-2 receptor expressed on activated T cells. This product can be used for research purposes.

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Lab products found in correlation

Lsrii system, by BD (2 mentions) Sytox blue, by Thermo Fisher Scientific (2 mentions) Diva software, by BD (2 mentions) Anti cd19 6d5, by BioLegend (1 mentions) Celltrace cfse proliferation kit, by Thermo Fisher Scientific (1 mentions) Cytofix cytoperm fixation permeabilization kit, by BD (1 mentions) Mouse igg2b, by BD (1 mentions)

Spelling variants of this product

CD25 (PC61, (19 citations) Anti-CD25 (PC61, (13 citations)

2 protocols using anti cd25 pc61.5

T-cell Phenotyping by Flow Cytometry

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Stained cells were analyzed using an LSRII system (BD Biosciences). Data were analyzed with a Diva software (BD Biosciences). Cell viability was evaluated using SYTOX Blue (Life Technologies). The following antibodies were used: anti-CD5 (53-7.3), anti-CD4 (RM4-5), anti-CD8α (53-6.7), anti-TCRγ (GL-3), anti-TCRβ (H57-597), all from BD Biosciences; anti-CD25 (PC61.5), and anti-CD19 (6D5) both from BioLegend. A CellTrace CFSE proliferation kit (Molecular Probes) was used.

Roncagalli R., Hauri S., Fiore F., Liang Y., Chen Z., Sansoni A., Kanduri K., Joly R., Malzac A., Lähdesmäki H., Lahesmaa R., Yamasaki S., Saito T., Malissen M., Aebersold R., Gstaiger M, & Malissen B. (2014). Quantitative proteomic analysis of signalosome dynamics in primary T cells identifies the CD6 surface receptor as a Lat-independent TCR signaling hub. Nature immunology, 15(4), 384-392.

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Multiparametric Flow Cytometry Analysis

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The following antibodies were used: anti-CD5 (53–7.3), anti-CD4 (RM4-5), anti-CD8α (53–6.7), anti-TCRβ (H57-597), anti-CD44 (IM7), anti-CD3 (145-2C11), anti-CD24 (M1/69), anti-Vα2 (B20-1) all from BD Biosciences and anti-CD25 (PC61.5) from BioLegend. Cell viability was evaluated using SYTOX Blue (Life Technologies). For intracellular staining of LCK, cells were permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit according to manufacturer protocol. Anti-LCK antibody (clone 3A5; Santa Cruz) and an isotype control antibody (mouse IgG2b from BD Biosciences) were used at 10 μg/ml. A FITC-conjugated goat anti-mouse IgG2b secondary antibody (Southern Biotechnology) was used to reveal the primary antibody. Stained cells were analysed using an LSRII system (BD Biosciences). Data were analysed with Diva software (BD Biosciences), and overlayed plots were constructed with FlowJo software.

Paster W., Bruger A.M., Katsch K., Grégoire C., Roncagalli R., Fu G., Gascoigne N.R., Nika K., Cohnen A., Feller S.M., Simister P.C., Molder K.C., Cordoba S.P., Dushek O., Malissen B, & Acuto O. (2014). A THEMIS:SHP1 complex promotes T-cell survival. The EMBO Journal, 34(3), 393-409.

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