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2 protocols using 4 chloro 7 nitrobenzofurazane

1

Protein Characterization via Sulfhydryl Assays

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Lysozyme from chicken egg white (about 100,000 U/mg), α-chymotrypsinogen A from a bovine pancreas, ribonuclease A from a bovine pancreas (Type XII-A, 75–125 Kunitz units/mg protein), l-cysteine, cysteamine, l-glutathione, oxidized glutathione, N-acetyl-l-cysteine, cysteinylglycine, l-cysteine ethyl ester, 1-chloro-2,4-dinitrobenzene, 5,5′-dithiobis(2-nitrobenzoic acid), 4-chloro-7-nitrobenzofurazane, dithiotreitol, ethylendiamminotetreaacetic acid, urea, 8-anilinonaphthalene-1-sulfonic acid and all of the other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Enzymatic Characterization of Lysozyme

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Lysozyme (Lyz) from chicken egg white (about 100,000 U/mg), L-cysteine (Cys), L-cystine, L-glutathione (GSH), oxidized glutathione (GSSG), cysteamine, cystamine, 1-chloro-2,4-dinitrobenzene (CDNB), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), 4-chloro-7-nitrobenzofurazane (NBD-Cl), dithiotreitol (DTT), bromopyruvic acid, lysozyme activity kit, pepsin A and all other reagents were from SIGMA-Aldrich (St. Louis, Mo, USA).
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