Goat anti influenza np antibody

Paraffin embedded lung sections were subjected to antigen retrieval in boiling citrate buffer and stained with polyclonal goat anti-influenza NP antibody or goat IgG isotype control (Abcam), followed by biotin-conjugated donkey anti-goat secondary antibody (Jackson Immunoresearch). Staining was developed using the Vectastain ABC kit and DAB substrate (Vector Laboratories) as per manufacturer’s instructions. The extent of bronchiolar NP staining was semi-quantitatively analyzed by a blinded investigator, using the following scoring system. The mean of all airways in one section (≥10) was reported for each mouse.0, No NP staining. 1, 1-25 % of epithelial cells in airway stained. 2, 26-50 % of epithelial cells in airway stained. 3, 51-75 % of epithelial cells in airway stained. 4, 51-75 % of epithelial cells in airway stained. An additional +1 score was added where one or more patches of dense staining was observed, giving a maximum score of 5 per bronchiole.