Online ultraviolet detector

Manufactured by Shimadzu

The Shimadzu online ultraviolet-detector is a laboratory instrument designed to continuously monitor and detect the presence of ultraviolet light absorption in liquid samples. It provides real-time measurement of UV absorbance without the need for manual sample handling.

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Lab products found in correlation

Optilab t rex refractometer, by Wyatt Technology (2 mentions) Astra 6, by Wyatt Technology (1 mentions) Superdex 200 increase 10 300 gl column, by GE Healthcare (1 mentions) Minidawn treos instrument, by Wyatt Technology (1 mentions) Superdex 75 increase 10 300 gl size exclusion column, by GE Healthcare (1 mentions) Minidawn treos, by Wyatt Technology (1 mentions)

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Ultraviolet detector (8 citations)

2 protocols using online ultraviolet detector

Size-Exclusion Chromatography for Protein Characterization

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Protein samples (100 μl) were injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), with HBS pH 7.4 as running buffer at 0.5 ml min⁻¹, coupled to an online ultraviolet-detector (Shimadzu), a multi-angle light scattering miniDAWN TREOS instrument (Wyatt) and a Optilab T-rEX refractometer (Wyatt) at 25 °C. A refractive index increment (dn/dc) value of 0.185 ml g⁻¹ was used for protein concentration and molecular mass determination. Data were analysed using the ASTRA6 software (Wyatt). Correction for band broadening was applied using parameters derived from BSA injected under identical running conditions. For the analysis of TSLP-traps, conjugate analysis was performed using theoretical protein extinction coefficients and a dn/dc-value of 0.160 ml g⁻¹ for the glycan modifier.

Verstraete K., Peelman F., Braun H., Lopez J., Van Rompaey D., Dansercoer A., Vandenberghe I., Pauwels K., Tavernier J., Lambrecht B.N., Hammad H., De Winter H., Beyaert R., Lippens G, & Savvides S.N. (2017). Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nature Communications, 8, 14937.

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Size Exclusion Chromatography of His-tagged Proteins

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Purified His-tagged proteins anchor domain (2.1 mg/ml) was injected onto a Superdex 75 Increase 10/300 GL size exclusion column (GE Healthcare) and equilibrated with 20 mM Hepes (pH 7.4), 150 mM NaCl, and 1 mM TCEP coupled to an online ultraviolet detector (Shimadzu), a mini DAWN TREOS (Wyatt) multiangle laser light scattering detector, and an Optilab T-rEX refractometer (Wyatt) at room temperature. A refractive index increment (dn/dc) value of 0.185 ml/g was used. Band broadening corrections were applied using parameters derived from ribonuclease injected under identical running conditions. Data analysis was carried out using the ASTRA6.1 software.

Yperman K., Wang J., Eeckhout D., Winkler J., Vu L.D., Vandorpe M., Grones P., Mylle E., Kraus M., Merceron R., Nolf J., Mor E., De Bruyn P., Loris R., Potocký M., Savvides S.N., De Rybel B., De Jaeger G., Van Damme D, & Pleskot R. (2021). Molecular architecture of the endocytic TPLATE complex. Science Advances, 7(9), eabe7999.

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