β nicotinamide adenine dinucleotide 2 phosphate reduced tetrasodium salt hydrate nadph

All chemicals and biochemicals reagents were purchased from Sigma-Aldrich (Saint Louis, MO, USA) except RPMI 1640 medium and l-glutamine which were from Lonza (Walkersville, MD, USA), fetal bovine serum (FBS) was from Gibco BRL (Cergy Pontoise, France), Bradford reagent was obtained from Bio-Rad (Hercules, CA, USA) and β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH) was purchased from Roche Diagnostics (Mannheim, Germany). The human breast cancer cell lines (MDA-MB-468 and MCF-7) were from the stock cultures of the Laboratory of Experimental Oncology and Natural Substances, Faculty of Sciences & Technology, Sultan Moulay Slimane University (Beni-Mellal, Morocco).

RPMI 1640 medium and L-Glutamine were purchased from Lonza, Fetal Bovine Serum was from Gibco BRL (Cergy Pontoise, France), Bradford reagent was obtained from Bio-Rad (Hercules, CA, USA), and β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH) from Roche Diagnostics (Mannheim, Germany). ProtoDioscin and Dioscin natural compounds tested in this study were purchased from Sigma Aldrich, and their purity was ≥ 99%. Cepltatin (Cisplatine) was obtained from Pharmedic Laboratories Pvt (Ltd.) Pakistan. All other chemicals and biochemical reagents were purchased from Sigma-Aldrich. Two human breast adenocarcinomas cell lines came from the Curie Institute bank (Translational Research Department, Breast Cancer Biology Team, Paris, France). They were subsequently obtained from the parent cultures of the laboratory of Experimental Oncology and Natural Substances of the Faculty of Science and Technology of Sultan Moulay Slimane University, Beni Mellal.