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Milliplex map multiplex human cytokine panel 1

Manufactured by Merck Group

The MILLIPLEX MAP multiplex Human Cytokine Panel 1 is a laboratory equipment product designed for the detection and quantification of multiple human cytokines in a single sample. The product utilizes the multiplex technology to simultaneously measure the levels of various cytokines.

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2 protocols using milliplex map multiplex human cytokine panel 1

1

Multiplex Cytokine Profiling of PRP

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We performed magnetic bead-based sandwich immunoassays using the MILLIPLEX MAP multiplex Human Cytokine Panel 1 (cat #HCYTOMAG-60 k; EMD-Millipore Corporation, St. Charles, MO) following the manufacturer’s instructions. Duplicate wells of activated PRP samples were analyzed using a Luminex MagPix (Luminex). Cytokine concentrations were determined using the Luminex Xponent 4.2 and EMD-Millipore Milliplex Analyst v5.1. A human TGFb1 Quantikine ELISA Kit (R&D Systems) was used to evaluate TGFβ1 in PRP, using a Tecan Infinite M200 PRO plate reader, with the accompanying i-control 1.12 software (Tecan).
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2

Multiplex Cytokine Quantification

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Magnetic bead-based sandwich immunoassays for cytokines using MILLIPLEX MAP multiplex Human Cytokine Panel 1 (cat #HCYTOMAG-60k; EMD-Millipore Corporation, St. Charles, MO) were performed according to the manufacturer’s instruction. Duplicate wells of cell culture supernatant samples (25 μl) were analyzed by Luminex MagPix (Luminex Corp, Austin, TX). Cytokine concentrations were determined by Luminex Xponent 4.2 and EMD-Millipore Milliplex Analyst v5.1 using 5-p log analysis.
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