Agilent 1290 l

UPLC-Q-TOF-MS/MS analysis of the EA fraction from P. sibiricum was carried out by ultra-high-performance liquid chromatography (UPLC) coupled to quadrupole-flight mass spectrometry (Q-TOF-MS/MS, Agilent 1290 L, Agilent 6530 MS, Agilent Technologies, Santa Clara, CA, USA). The chromatographic separation was carried out on a Sunniest C18 ACQ (2.1 mm × 50 mm, 1.7 μm) column at 30 °C, with the mobile phase consisting of ultrapure water containing 0.1 percent formic acid (A) and ACN (B). The Q-TOF-MS analysis was performed in the negative-ion modes with a dual ESI source. The LC elution gradient was set as follows: 0–20 min, 5–20% B; 20–30 min, 20–30% B; 30–40 min, 30–50% B. The flow rate was then 0.2 mL/min, and the injection volume was 20 µL. The following MS parameters were set: The capillary voltage (Vcap) was 3500 V and the fragmentor voltage was 175 V, respectively. The capillary temperature was 350 °C, and the drying gas flow rate was 8 L/min. The pressure in the nebulizer was set at 35 psi. The fixed collision energies were set as 10, 20, 40 and 60 V. The Mass Hunter workstation (Agilent) with a mass range of m/z 100–1500 was used to obtain profile data at a rate of one spectrum per second. The compounds were identified by comparing their retention times, parent ions, and mass fragments with references and databases.

UPLC-Q-TOF-MS/MS analysis of EA fraction from A. indica was performed by ultra-high-performance liquid chromatography (UPLC) coupled to quadrupole-flight mass spectrometry (Q-TOF-MS/MS, Agilent 1290 L, Agilent 6530 MS, Agilent Technologies, Santa Clara, CA, USA). A Sunniest C18 HT (2.1 mm × 100 mm, 2 µm) column was used for the chromatographic separation at 25 °C. The Q-TOF-MS analysis was conducted with a dual ESI source in the negative-ion modes. The ultrapure water (A, 0.1% formic acid) and acetonitrile (B, ACN) were used, and the flow rate was 0.2 mL/min. The injection volume was 20 µL and the LC elution gradient was set as follows: 0–3 min, 5–10% B; 3–8 min, 10–10% B; 8–20 min, 10–23% B; 20–30 min, 23–30% B; 30–40 min, 30–60% B; 40–45 min, 60–95% B. The MS parameters were set as follows: the capillary voltage (Vcap) was 3500 V and fragmentor voltage was 175 V. The capillary temperature and drying gas flow rate were 350 °C and 8 L/min, respectively. The nebulizer pressure was set at 35 psi. The fixed collision energies were set as 10, 20, and 40 V. Mass Hunter workstation (Agilent) with a mass range of m/z 100–1100 at a rate of 1 spectra per second was utilized to obtain profile data. Compounds were identified by comparing their retention time, parent ions and mass fragments with references and databases.