Total protein cell extracts were prepared using a cell lysis buffer (Cell Signaling Technology, Inc., Danvers, MA, USA) with 1 mM PMSF and Protease Inhibitor Cocktail 1/200 v/v (Sigma-Aldrich). Protein were separated by SDS-PAGE, transferred onto nitrocellulose membrane Amersham Protran (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) and analyzed by standard Western blotting using rabbit polyclonal anti-GDAP1 (Abcam, Cambridge, MA, USA), purified mouse anti-GM130 (BD Transduction Laboratories, San Jose, CA, USA), rabbit polyclonal anti-B4GALT3 (Proteintech, Chicago, IL, USA), rabbit polyclonal anti-GORASP2 (Proteintech), rabbit anti-TGN46 (Sigma-Aldrich), mouse monoclonal anti-beta Actin (Proteintech) antibodies and secondary anti-rabbit IgG and anti-mouse IgG horseradish peroxidase (HRP)-conjugated antibodies (Sigma-Aldrich) followed by enhanced chemiluminescence (Western Bright Sirius Advansta, San Jose, CA, USA).
Purified mouse anti gm130
Manufactured by BD
Sourced in United States
Purified mouse anti-GM130 is a laboratory reagent used in research applications. It is an antibody that recognizes the GM130 protein, which is a component of the Golgi apparatus in cells. The primary function of this product is to enable the detection and study of the GM130 protein in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Proteintech (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
Anti gdap1, by Abcam (1 mentions)
Amersham protran, by GE Healthcare (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Alkaline phosphatase substrate kit, by Merck Group (1 mentions)
Protein g agarose bead, by Santa Cruz Biotechnology (1 mentions)
Proteostat aggresome detection kit, by Enzo Life Sciences (1 mentions)
Anti rabbit igg hrp, by GE Healthcare (1 mentions)
Polyclonal anti flag, by Merck Group (1 mentions)
Anti gapdh polyclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using purified mouse anti gm130
1
Protein Extraction and Western Blotting
2
Antibody Immunoassay Reagents
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Monoclonal anti-HA (3F10) antibody was from Roche Applied Science (Pleasanton, CA). Anti-GAPDH polyclonal antibody, anti–TCP-1η (CCT7) (E-20) goat polyclonal antibody, anti-β2AR (H-73) polyclonal antibody, and protein G–agarose beads were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Texas Red anti-goat immunoglobulin G (IgG) was from Jackson ImmunoResearch (West Grove, PA). Alexa Fluor 488, Alexa Fluor 633 anti-rabbit IgG, and ProLong Gold antifade reagent were purchased from Invitrogen (Carlsbad, CA). Anti–c-Myc (9E10) monoclonal antibody was from Covance (Montreal, Canada). Anti–Myc-horseradish (HRP) polyclonal antibody was from Abcam (Toronto, Canada). Anti–rabbit-HRP IgG and anti–mouse-HRP IgG were purchased from GE Healthcare (Baie d'Urfé, Canada). Anti–goat-HRP IgG was from R&D Systems (Minneapolis, MN). Polyclonal anti-FLAG, the alkaline phosphatase–conjugated anti-mouse antibodies, and the alkaline phosphatase substrate kit were purchased from Sigma-Aldrich (Oakville, Canada). PROTEOSTAT Aggresome Detection Kit was from Enzo Life Sciences (Farmingdale, NY). Goat anti–GST-HRP antibody was purchased from Bethyl Laboratories (Montgomery, TX). Purified mouse anti-GM130 was purchased from BD Transduction Laboratories (San Jose, CA).
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