Anti cd163 clone10d6

Immunohistochemical studies were performed on formalin-fixed, paraffin-embedded sections as previously described [19 (link), 40 (link)], or in a Dako AutoStainer (Dako, Carpinteria, CA). Antibodies used include: anti-CD68 (clone PG-M1, Dako, dilution 1:100); anti-CD163 (clone10D6, Biocare Medical, Concord, CA, USA, dilution 1:50); anti-CD31 (clone JC70A, Dako, ready-to use); anti-MMP-9 (clone Ab-2, Oncogene Research Products Cambridge, MA, dilution 1:50); and anti E-cadherin (Dako, clone NCH-38, ready to use).

Immunohistochemical analysis was carried out on 3-μm thick paraffin sections as described [12 (link)]. Antibodies used include: anti-ERα (clone 6F11, Santa Cruz Biotechnology, dilution 1:100); anti-ERβ1 (clone PPG5/10, Dako, Glostrup, Denmark, dilution 1:50); anti-ERβ2 (clone 57/3, Serotec Ltd, dilution 1:200); anti-ERβ5 (clone 5/25, Serotec Ltd, dilution 1:300); anti-PR (clone 16SAN27, Leica Microsystems, dilution 1:100); anti-GPER (clone N-15, Santa Cruz, dilution 1:100); anti-Ki67 (clone MIB-1, Dako, dilution 1:100); anti-CD31 (ab28364, Abcam, dilution 1:50), anti-CD11b (Thermo Fisher scientific, Waltham, MA, USA, dilution 1:50); anti-arginase I (Santa Cruz Biotechnology, dilution 1:50); anti-CD68 (clone PG-M1, Dako, dilution 1:100) or anti CD163 (clone10D6, Biocare Medical, Concord, CA, USA, dilution 1:50).