Log In Sign up
The largest database of trusted experimental protocols

Anti mta1

Manufactured by Abcam
Visit Supplier
Sourced in United Kingdom

Anti-MTA1 is a protein detection reagent used to identify the presence of the MTA1 (Metastasis Associated 1) protein in biological samples. It is an antibody that specifically binds to the MTA1 protein, allowing for its detection and measurement.

Automatically generated - may contain errors

Lab products found in correlation

Anti hdac2, by Abcam (2 mentions) Anti tpr, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Bioworld Technology (1 mentions) Anti histone h3, by Bioworld Technology (1 mentions) Molecular imager chemidoc xrs system, by Bio-Rad (1 mentions) Pierce ecl substrate western blot detection system, by Thermo Fisher Scientific (1 mentions) Anti e cadherin, by Abcam (1 mentions) Lactic acid, by Merck Group (1 mentions) Tunel assay kit, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Anti cd45, by BioLegend (1 mentions) Anti foxp3, by BioLegend (1 mentions) Anti tgf β, by Abcam (1 mentions) Anti hmgb1, by Abcam (1 mentions) Anti crt, by Abcam (1 mentions) Anti cd34, by Abcam (1 mentions) Glycolic acid, by Merck Group (1 mentions) Stannous octoate, by Merck Group (1 mentions) Anti cd3, by BioLegend (1 mentions) Anti cd86, by BioLegend (1 mentions)

4 protocols using anti mta1

1

Antibody Validation for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: mouse monoclonal antibody anti-MTA1 (Abcam); rabbit polyclonal antibody anti-MTA1 (Abcam); mouse monoclonal antibody anti-HDAC2 (Abcam); rabbit polyclonal antibody anti- HDAC2 (Bioworld Technology); rabbit polyclonal antibody anti-GAPDH (Bioworld Technology); rabbit polyclonal antibody anti-histone H3 (Bioworld Technology); and rabbit polyclonal antibody anti-TPR (Santa Cruz).
Liu J., Xu D., Wang H., Zhang Y., Chang Y., Zhang J., Wang J., Li C., Liu H., Zhao M., Lin C., Zhan Q., Huang C, & Qian H. (2014). The subcellular distribution and function of MTA1 in cancer differentiation. Oncotarget, 5(13), 5153-5164.
+ Open protocol
+ Expand
2

Western Blot Analysis of Epithelial Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted from cultured cells, quantitated using a protein assay (bicinchoninic acid [BCA] method; Beyotime, Shanghai, China). Proteins were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, transferred to polyvinylidene fluoride (PVDF) membrane, blocked in 4 % dry milk at room temperature for 1 hour, and immunostained with primary antibodies at 4 °C over-night using anti-MTA1 (1:2000, Abcam, Cambridge, MA), anti-E-cadherin (1:1000; Abcam, Cambridge, MA), and anti-GAPDH (1:1000, Kangchen,China). The results were visualized via a chemiluminescent detection system (Pierce ECL Substrate Western blot detection system; Thermo, Rockford, IL) and exposed in Molecular Imager ChemiDoc XRS System (Bio-Rad, Hercules, CA).
Xu C., Hua F., Chen Y., Huang H., Ye W., Yu Y, & Shen Z. (2015). MTA1 promotes metastasis of MPM via suppression of E-cadherin. Journal of Experimental & Clinical Cancer Research : CR, 34, 151.
+ Open protocol
+ Expand
3

Multifunctional Nanoparticle Delivery System

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lactic acid (LA), glycolic acid (GA), stannous octoate, hydroxyl-terminated PEG, combretastatin A4 disodium phosphate (CA4P) and epirubicin (EPI) were purchased from Sigma–Aldrich (USA). Primary antibodies, including anti-PCNA, anti-CD34, anti-CD31, anti-MTA1, anti-TGF-β, anti-CRT and anti-HMGB1, were obtained from Abcam (Cambridge, UK). Antibodies for flow cytometric analysis, including anti-CD45, anti-CD11c, anti-CD86, anti-CD11b, anti-Gr-1, anti-CD3, anti-CD4, anti-CD8 and anti-FOXP3 were purchased from Biolegend. The 4′,6-diamidino-2-phenylindole (DAPI) and TUNEL assay kit were purchased from Thermo Fisher Scientific, Waltham, MA, USA.
Li F., Shao X., Liu D., Jiao X., Yang X., Yang W, & Liu X. (2022). Vascular Disruptive Hydrogel Platform for Enhanced Chemotherapy and Anti-Angiogenesis through Alleviation of Immune Surveillance. Pharmaceutics, 14(9), 1809.
+ Open protocol
+ Expand
4

Chromatin Immunoprecipitation Profiling Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following commercially available antibodies were used:
Anti-H3 (1:5.000, #61475), Anti-H3K36me3 (1:4.000, #61102), Anti-H3K36me2 (1:1.000, #39255), Anti-H3K36me1 (1:1.000, #61351), Anti-H3K4me3 (1:500, #61379) and anti-H2A (1:1.000, #39209) were from Active Motif. Anti-H3K27me3 (1:1.000, C15410069), Anti-H3K79me3 (1:1.000, C15310068), anti-H2A.Zac (2 µg/IP, C15410202-050) and Anti-H3K9me3 (1:1.000, C15410056) were from Diagenode. Anti-H4K20me3 (1:1.000, ab9053), Anti-H3 (1:30.000, ab1791), anti-MTA1 (1:1.000, ab71153 or 3 µg/IP, ab50209), anti-CHD4 (1:1.000 or 2 µg/IP, ab70469), anti-HDAC2 (1:5.000, ab124974) anti-RBBP4 (1:1.000, ab488), anti-RBBP7 (1:1.000, ab3535), Anti-H3K27ac (2 µg/IP, ab4729), anti-H2A.Z (1:3.000, ab4174), and anti-MBD3 (1:5.000, ab157464) were from Abcam. Anti-PWWP2A (1:1.000, NBP2-13833) from Novus (Acris). Anti-HA-HRP (1:40.000, 2999S) and anti-HDAC1 (1:20.000, 5356S) from Cell Signaling Technology. Anti-FLAG M2 (1:80.000 or 3 µg/IP, F1804) and anti-mouse IgG (3 µg/IP, I8765) from Sigma-Aldrich.
The following secondary antibodies were used:
Anti-rabbit IRDye 800CW (1:10.000, 926-32211) and anti-mouse IRDye 680RD (1:10.000 926‐68070) from LI-COR Biosciences. Anti-mouse-HRP (1:10.000, M114) from Leinco Technologies. Anti-mouse and anti-rabbit HRP-linked antibodies (1:10.000, NA931 and NA934) from VWR.
Link S., Spitzer R.M., Sana M., Torrado M., Völker-Albert M.C., Keilhauer E.C., Burgold T., Pünzeler S., Low J.K., Lindström I., Nist A., Regnard C., Stiewe T., Hendrich B., Imhof A., Mann M., Mackay J.P., Bartkuhn M, & Hake S.B. (2018). PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nature Communications, 9, 4300.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!