Anti spop antibody

In general, total antibodies were used at a 1:2000 dilution in 5% skim milk in 1× phosphate-buffered saline (PBS), and phosphoantibodies were used at a 1:1000 dilution in 3% bovine serum albumin (BSA) in 1× PBS for western blotting (WB). Anti-ELK3 (cat. no.: NBP2-01264) antibody was purchased from Novus (St. Louis, MO, USA). The anti-SPOP antibody was purchased from Proteintech (cat. no.: 16750-1-AP, Rosemont, IL, USA). Anti-HA agarose beads were purchased from Sigma-Aldrich (cat. no.: A2095, St Louis, MO, USA). Anti-Xpress (cat. no.: R910-25) antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-Flag (cat. no.: M185-3), anti-Flag-HRP (cat. no.: M185-7), and anti-HA-HRP (cat. no.: M180-7) antibodies were purchased from MBL. Anti-c-Myc (cat. no.: SC-40) and β-actin (cat. no.: SC-47778) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-Cul3 (cat. no.: 2759), anti-pT68-CHK2 (cat. no.: 2197), anti-CHK2 (cat. no.: 6334), anti-pS345-CHK1 (cat. no.: 2348), anti-CHK1 (cat. no.: 2360), Cyclin E (cat. no.: 4129), anti-phospho-Akt substrate (cat. no.: 10001), anti-c-Fos (cat. no.: 2250) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).