Anti chicken igy alkaline phosphatase antibody

Manufactured by Merck Group

Sourced in United Kingdom

The Anti-Chicken IgY−Alkaline Phosphatase (AP) antibody is a laboratory reagent used for the detection and quantification of chicken immunoglobulin Y (IgY) in various analytical and research applications. The antibody is conjugated with the enzyme Alkaline Phosphatase, which serves as a reporter molecule to facilitate the visualization and measurement of target IgY proteins.

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Lab products found in correlation

Bradford reagent, by Merck Group (2 mentions) Thiophilic gel chromatography, by Thermo Fisher Scientific (2 mentions) Melon gel igg purification kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Alkaline phosphatase (284 citations) Phosphatases (7 citations) Anti-Chicken IgY antibody (2 citations)

2 protocols using anti chicken igy alkaline phosphatase antibody

Purification of Porcine IgG and Chicken IgY

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Porcine IgG was purified using a Melon™ Gel IgG Purification Kit (Thermo-Scientific,UK) following the manufacturer’s instructions. After purification of sera (50 μl) the eluate (500 μl) was stored at 4 °C after the addition of NaCl to a final concentration of 100 mM. Protein content was estimated using a Bradford reagent (Sigma-Aldrich, UK). Chicken IgY was purified by Thiophilic gel chromatography (Thermo-Scientific, UK), exactly as described previously30 , eluted fractions were assayed by ELISA using Anti-Chicken IgY−Alkaline Phosphatase (AP) antibody produced in rabbit (Sigma-Aldrich, UK) and fractions containing IgY were pooled, protein content was estimated using a Bradford reagent (Sigma-Aldrich, UK) and samples stored at 4 °C.

Naqid I.A., Owen J.P., Maddison B.C., Spiliotopoulos A., Emes R.D., Warry A., Tchórzewska M.A., Martelli F., Gosling R.J., Davies R.H., La Ragione R.M, & Gough K.C. (2016). Mapping polyclonal antibody responses to bacterial infection using next generation phage display. Scientific Reports, 6, 24232.

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Chicken IgY Purification by Thiophilic Gel

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Chicken IgY was purified by Thiophilic gel chromatography (Thermo-Scientific, UK)19 . Eluted fractions were assayed by ELISA using Anti-Chicken IgY-Alkaline Phosphatase (AP) antibody produced in rabbit (Sigma-Aldrich, UK) and fractions containing IgY were pooled. Protein content was estimated using a Bradford reagent (Sigma-Aldrich, UK) before sample storage at 4 °C.

Naqid I.A., Owen J.P., Maddison B.C., Spiliotopoulos A., Emes R.D., Warry A., Flynn R.J., Martelli F., Gosling R.J., Davies R.H., La Ragione R.M, & Gough K.C. (2016). Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals. Scientific Reports, 6, 31186.

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