Mass spectra were acquired under linear and reflectron modes, using a Bruker Ultraflex II MALDI-TOF/ TOF controlled by FLEXCONTROL 3.0 software (Bruker Daltonics, Bremen, Germany). External calibration was performed while using Peptide Standard Calibration II (Bruker Daltonics) and Peptide Calibration Mix 3 (PepMix3; New England Biolabs, Ipswich, MA, USA) for reflection and linear modes, respectively. The matrix, α-cyano-4-hydroxycinnamic acid (Sigma-Aldrich, St. Louis, MO, USA) was prepared at a concentration of 20 mg·mL−1 in 1:1 acetonitrile containing 0.1% TFA solution. AbeTx1 solution (1μL) dropped onto the MALDI sample plate (384 positions; Bruker Daltonics) was added to the matrix solution (1μL) and dried at room temperature. Spectra were analyzed using thebFLEXANALYSIS 3.0 program (Bruker Daltonics Software).
Maldi sample plate
Manufactured by Bruker
The MALDI sample plate is a specialized component used in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It serves as the platform for holding and presenting samples for analysis in the MALDI process.
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2 protocols using maldi sample plate
1
MALDI-TOF/TOF Mass Spectrometry Analysis
2
MALDI-TOF MS Sample Preparation and Analysis
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We prepared diluted solutions for matrix-assisted laser desorption/ionization TOF MS (MALDI-TOF MS) by mixing 1 μl each of diluted samples with 0.5 μl of matrix solution containing 2 g/L α-cyano-4-hydroxycinnamic acid, 10 nmol/L angiotensin II, 10 nmol/L adrenocorticotropic hormone 18–39 (Bruker Daltonics), and 10 ml/L formic acid in 500 ml/L acetonitrile and allowing each droplet to dry on the MALDI sample plate (Bruker Daltonics). We used an Auto Flex II MALDI-TOF mass spectrometer (Bruker Daltonics), operated in positive ion linear or reflectron mode. Briefly, all spectra were processed by automatic baseline subtraction, peak detection, recalibration, and peak area calculation, according to the predefined settings.
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