The levels of total cholesterol (TC), triglyceride (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and γ-glutamyl transpeptidase (γ-GT) in serum were accessed by an automatic biochemistry analyser (Hitachi, Japan) according to the manufacturer’s instructions. In addition, the levels of TC, TG, AST, ALT, and γ-GT in liver tissues were detected using Total cholesterol assay kit (Nanjing Jiancheng Bioengineering Institute, China), Triglyceride assay kit (Nanjing Jiancheng Bioengineering Institute, China), Aspartate aminotransferase Assay Kit (Nanjing Jiancheng Bioengineering Institute, China), Alanine aminotransferase Assay Kit (Nanjing Jiancheng Bioengineering Institute, China), and γ-glutamyl transferase Assay Kit (Nanjing Jiancheng Bioengineering Institute, China), respectively.
Automatic biochemistry analyser
Manufactured by Hitachi
Sourced in Japan
The Automatic Biochemistry Analyser is a laboratory instrument designed for the automated analysis of biochemical samples. It is capable of performing a variety of tests on biological fluids, such as blood, urine, or other bodily substances, to measure the levels of various chemical compounds and biomarkers. The core function of this analyser is to provide accurate and efficient quantitative analysis of these analytes to aid in clinical diagnostics and research.
Automatically generated - may contain errors
Lab products found in correlation
Triglyceride assay kit, by Nanjing Jiancheng (1 mentions)
γ glutamyl transferase assay kit, by Nanjing Jiancheng (1 mentions)
Total cholesterol assay kit, by Nanjing Jiancheng (1 mentions)
Alanine aminotransferase assay kit, by Nanjing Jiancheng (1 mentions)
Aspartate aminotransferase assay kit, by Nanjing Jiancheng (1 mentions)
Cobas amplicor hbv monitor test, by Roche (1 mentions)
5 protocols using automatic biochemistry analyser
1
Serum and Liver Biochemical Assays
2
Comprehensive HBV Screening Protocol
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Peripheral blood samples were collected from all participants before the initiation of PEG-IFN α-2a treatment. HBV serological markers (HBsAg, HBeAg, anti-HBs antibody, anti-HBe antibody, anti-HBc IgM and IgG) were detected using the Chemiluminescent Microparticle Immunoassay (Abbott Ireland, Sligo, Ireland). HBV DNA was quantified using quantitative PCR (Roche, Basel, Switzerland) with the detection range from 2.7 log10 to 8 log10 IU/mL. Biochemical tests, including serum ALT levels, were measured using an automatic biochemistry analyser (Hitachi, Tokyo, Japan).
3
Liver Biochemistry and HBV Markers
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The liver biochemistry was measured by standard laboratory procedures with an automatic biochemistry analyser (Hitachi). HBV serological markers, including HBsAg, HBeAg, and antibody against HBsAg (HBsAb) and against HBeAg (HBeAb), were determined with a chemiluminescence microparticle enzyme immunoassay (Abbott). HBV DNA levels were determined with the Cobas Amplicor HBV monitor test (Roche Diagnostics), with a lower detection limit of 60 IU ml−1.
4
Anthropometric and Metabolic Measurements
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Anthropometric measurements, including height and weight, waist circumference (WC) were taken by welltrained examiners, with subjects wearing light, thin clothing and no shoes. Body weight and height were measured to the nearest 0.1 kg and 0.1 cm, respectively. BMI was calculated as weight (kg) divided by the square of the height in metres (m 2 ). Fasting and postprandial (2 hours after oral 75 g glucose loading) blood samples were taken from all subjects at baseline and follow-up. Fasting blood glucose (FBG) and 2-hour postprandial glucose (2-h PG), blood lipids including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c) and high-density lipoprotein cholesterol (HDL-c) were measured using an automatic biochemistry analyser (Hitachi, Tokyo, Japan).
5
Fasting Blood Tests for Lipid and Kidney Assessment
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Blood samples were collected in the morning after overnight fasting and were evaluated biochemical and clinical parameters on the same day. FBG was measured by an oxidase enzymatic method. The levels of high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC) and triglyceride (TG) were assessed enzymatically using commercial reagents with an automatic biochemistry analyser (Hitachi, Tokyo, Japan). The levels of low-density lipoprotein cholesterol (LDL-C) were calculated by the Friedewald formula.11 (link) The estimated glomerular filtration rate (eGFR) was calculated on the basis of the chronic kidney disease-epidemiology creatinine equation12 (link).
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