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Cmyc primary antibody

Manufactured by Santa Cruz Biotechnology
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The CMYC primary antibody is a research-use only product intended for the detection of the c-Myc protein, a transcription factor that plays a crucial role in cell proliferation, growth, and apoptosis. This antibody is designed for use in various immunological techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to aid in the study of c-Myc expression and regulation in biological systems.

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Lab products found in correlation

Steamer set, by IHC World (2 mentions) Caseviewer software, by 3DHISTECH (2 mentions) Ihc tek epitope retrieval solution, by IHC World (2 mentions) Vector red alkaline phosphatase substrate kit 1, by Vector Laboratories (2 mentions) Harris modified hematoxylin, by Thermo Fisher Scientific (2 mentions) Fxr1 primary antibody, by Proteintech (2 mentions) Vectastain abc ap kit, by Vector Laboratories (2 mentions) Panoramic 250 flash 3 scanner, by 3DHISTECH (2 mentions)

2 protocols using cmyc primary antibody

1

Quantification of FXR1 and cMYC in Ovarian Cancer

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FXR1 and cMYC protein levels in human ovarian cancer tissues were analyzed using three TMAs (Cat# OV1005bt, Cat# OVC961 and Cat#OV1004, US Biomax Inc., Rockville, MD). For this purpose, the slides were dewaxed in xylene, and rehydrated through graded ethanol to distilled water. Antigen retrieval for the slide specimens were performed using IHC-Tek epitope retrieval solution and steamer set (IHC World, LLC.). The slides were then immersed in 3% H2O2 for 10 min to quench endogenous peroxidase followed by blocking with 10% goat serum for 1 h. Vectastain ABC-AP Kit (Vector Labs, Burlingame, CA) and Vector Red Alkaline Phosphatase Substrate Kit I (Vector Labs, Burlingame, CA) were used for tissue staining as per manufacture protocol. FXR1 primary antibody (Proteintech, Cat#13194-1-AP) was used at 1:200 dilution and cMYC primary antibody (Santacruz Biotechnology, Cat#sc-47694) at 1:100. Following, Vector red staining, the slides were counterstained with Harris modified hematoxylin (Thermo Fisher Scientific Inc., Rockford, IL), dehydrated with graded ethanol and xylene, and finally mounted with paramount. TMAs slides was digitally scanned using Panoramic 250 FLASH III scanner (3D HISTECH ltd. Version 2.0) and, using the Case Viewer software (3D HISTECH ltd. Version 2.0) was used to view and analyze images.
George J., Li Y., Kadamberi I.P., Parashar D., Tsaih S.W., Gupta P., Geethadevi A., Chen C., Ghosh C., Sun Y., Mittal S., Ramchandran R., Rui H., Lopez-Berestein G., Rodriguez-Aguayo C., Leone G., Rader J.S., Sood A.K., Dey M., Pradeep S, & Chaluvally-Raghavan P. (2021). RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. Cell reports, 37(5), 109934.
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2

Quantification of FXR1 and cMYC in Ovarian Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
FXR1 and cMYC protein levels in human ovarian cancer tissues were analyzed using three TMAs (Cat# OV1005bt, Cat# OVC961 and Cat#OV1004, US Biomax Inc., Rockville, MD). For this purpose, the slides were dewaxed in xylene, and rehydrated through graded ethanol to distilled water. Antigen retrieval for the slide specimens were performed using IHC-Tek epitope retrieval solution and steamer set (IHC World, LLC.). The slides were then immersed in 3% H2O2 for 10 min to quench endogenous peroxidase followed by blocking with 10% goat serum for 1 h. Vectastain ABC-AP Kit (Vector Labs, Burlingame, CA) and Vector Red Alkaline Phosphatase Substrate Kit I (Vector Labs, Burlingame, CA) were used for tissue staining as per manufacture protocol. FXR1 primary antibody (Proteintech, Cat#13194-1-AP) was used at 1:200 dilution and cMYC primary antibody (Santacruz Biotechnology, Cat#sc-47694) at 1:100. Following, Vector red staining, the slides were counterstained with Harris modified hematoxylin (Thermo Fisher Scientific Inc., Rockford, IL), dehydrated with graded ethanol and xylene, and finally mounted with paramount. TMAs slides was digitally scanned using Panoramic 250 FLASH III scanner (3D HISTECH ltd. Version 2.0) and, using the Case Viewer software (3D HISTECH ltd. Version 2.0) was used to view and analyze images.
George J., Li Y., Kadamberi I.P., Parashar D., Tsaih S.W., Gupta P., Geethadevi A., Chen C., Ghosh C., Sun Y., Mittal S., Ramchandran R., Rui H., Lopez-Berestein G., Rodriguez-Aguayo C., Leone G., Rader J.S., Sood A.K., Dey M., Pradeep S, & Chaluvally-Raghavan P. (2021). RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. Cell reports, 37(5), 109934.
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