Ab150424

Conventional protocols were used for IB with the details available elsewhere. The following primary antibodies were used for IB: anti-HERPUD1 (1:1000, ab150424, Abcam, Cambridge, UK), anti-GCLC (1:1000, ab190685, Abcam), anti-CBS (1:1000, ab140600, Abcam), anti-CTH (1:1000, ab189916, Abcam), anti-SHMT2 (1:1000, #33443, Cell Signaling Technology (CST), Boston, MA, USA), anti-GSS (1:1000, #ab124811, Abcam) or GSS (H-7) (1:500, sc-166882, Santa Cruz, Heidelberg, Germany), anti-MDM2 (1:1000, ab259265, Abcam), anti-SYVN1 (1:1000, #14773, CST), anti-GAPDH (1:1000, #5174, CST). The membranes were incubated with anti-rabbit IgG, HRP-linked antibody (1:2000, #7071, CST) or anti-mouse IgG, HRP-linked antibody (1:2000, #7076, CST) at room temperature for one hour, and visualized using Clarity Western ECL substrate. The relative expression levels of proteins were determined by densitometry using ImageJ software and were normalized to housekeeping protein.

The following primary antibodies were used: mouse monoclonal antibodies against MHV N and S proteins (kind gifts of Dr Helmut Wege, University of Würzburg), rabbit polyclonal anti-SARS-CoV-2 spike glycoprotein antibody (ab272504, Abcam) mouse anti-GAPDH (IgM specific, G8795, Sigma-Aldrich), mouse anti-Flag (F3165, Sigma-Aldrich), rabbit anti-HA (3724, Cell Signaling Technology), rabbit anti-PERK (ab229912, Abcam), rabbit anti-HERPUD1 (ab150424, Abcam), rabbit anti-GRP78 (BIP, ab108613, Abcam), rabbit anti-eIF2α (9722, Cell Signaling Technology), rabbit anti-phospho-eIF2α (Ser51, 9721, Cell Signaling Technology), rabbit anti-ATF4 (10835-1-AP, Proteintech), rabbit anti-ATF6 (ab203119 and ab37149, Abcam), mouse anti-S6 (2317, Cell Signaling Technology) and rabbit RPL10a (ab174318, Abcam). Secondary antibodies used for western blotting were purchased from Licor: IRDye 800CW Donkey Anti-Mouse IgG (H+L), IRDye 800CW Donkey Anti-Rabbit IgG (H+L), IRDye 680RD Goat Anti-Mouse IgG (H+L) and IRDye 680RD Goat Anti-Mouse IgM (μ chain specific).