Connexin 26

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Connexin-26 is a protein that forms gap junctions, allowing the passage of small molecules and ions between neighboring cells. It is a member of the connexin family of proteins and plays a role in cell-to-cell communication.

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2 protocols using connexin 26

Immunoblot Analysis of Signaling Proteins

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Cell lysate was prepared using lysis buffer consisting of 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 137 mM NaCl, 2.7 mM KCl, 0.5% deoxycholate, 1% NP-40, 0.3% SDS, and 1 mM phenylmethylsulfonyl fluoride.²⁹ (link) Immunoblot analyses were performed according to the procedure described previously.²⁹ (link) The antibodies used were specific for human STAT3, phosphorylated STAT3 (EMD Millipore, Billerica, MA, USA), HSP90α (AbD Serotec), phosphorylated FAK (Invitrogen), phosphorylated CDK2 (Epitomics, Burlingame, CA, USA), phosphorylated Rb, Rb, phosphorylated CDC2 (Cell Signaling, Danvers, MA, USA), connexin-43 (Sigma, St. Louis, MO, USA), integrin α_V (BD Biosciences, San Jose, CA, USA), cyclin D1, MMP-2, MMP-9 (Lab Vision/NeoMarkers Co., Fremont, CA, USA), CDK4, cyclin E, cyclin A, CDK2, cyclin B1, CDC2, FAK, TCF12, Twist-1, Snail, Slug, E-cadherin, connexin-26, fibronectin, and GAPDH (Santa Cruz Biotechnology).

Chen C.C., Chen L.L., Li C.P., Hsu Y.T., Jiang S.S., Fan C.S., Chua K.V., Huang S.X., Shyr Y.M., Chen L.T, & Huang T.S. (2018). Myeloid-derived macrophages and secreted HSP90α induce pancreatic ductal adenocarcinoma development. Oncoimmunology, 7(5), e1424612.

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Protein Expression Analysis of Cell Lysates

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After desired treatments, cells were lysed with RIPA buffer spiked with a fresh protease and phosphatase cocktail (Thermo Scientific, #78442) and sonicated. Protein concentrations were quantified using the Pierce BCA assay kit (Thermo Fisher, #23225). 80–120μg of protein for each sample was loaded onto SDS-PAGE gels, and then transferred onto PVDF membranes. The blots were incubated with the following antibodies: desmocollin 1 (sc-398590, RRID: AB_2894905), desmoglein 2 (sc-80663, RRID: AB_2093438), plakophilin (sc-33636, RRID: AB_2164139), connexin 26 (sc-7261, RRID: AB_2110895) and cFOS (sc-52, RRID: AB_2106783) from Santa Cruz; ER-α (#8644, RRID: AB_2617128), HA (#3724, RRID: AB_1549585), Non-phospho-β-catenin (#19807, RRID: AB_2650576), Histone H3 (#4499, RRID: AB_10544537), AIF (#5318, RRID: AB_10634755), GSK3β (Ser9, #5558, RRID: AB_10013750), phospho-GSK3α (Ser21, #9316, RRID: AB_659836), GSK3β (#12456, RRID: AB_2636978) and GSK3α (#4337, RRID: AB_10859910) from Cell Signaling Technology; β-catenin (#610154, RRID: AB_397555) from BD; Tubulin (T6557, RRID: AB_477584) and connexin 43 (C6219, RRID: AB_476857) from Sigma Aldrich; and TIMP3 (ab39184, RRID: AB_2204971) from Abcam.

Li Z., Wu Y., Yates M.E., Tasdemir N., Bahreini A., Chen J., Levine K.M., Priedigkeit N.M., Nasrazadani A., Ali S., Buluwela L., Arnesen S., Gertz J., Richer J.K., Troness B., El-Ashry D., Zhang Q., Gerratana L., Zhang Y., Cristofanilli M., Montanez M.A., Sundd P., Wallace C.T., Watkins S.C., Fumagalli C., Guerini-Rocco E., Zhu L., Tseng G.C., Wagle N., Carroll J.S., Jank P., Denkert C., Karsten M.M., Blohmer J.U., Park B.H., Lucas P.C., Atkinson J.M., Lee A.V, & Oesterreich S. (2022). Hotspot ESR1 mutations are multimodal and contextual modulators of breast cancer metastasis. Cancer research, 82(7), 1321-1339.

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