Western lightning enhanced chemiluminescence kit ecl

Manufactured by PerkinElmer

The Western Lightning enhanced chemiluminescence kit (ECL) is a laboratory equipment product designed to detect and quantify proteins through Western blot analysis. The kit uses a chemiluminescent substrate to produce a measurable light signal, which is proportional to the amount of the target protein present in the sample.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Western Lightning Plus-ECL Enhanced Chemiluminescence Substrate kit Western Lightning Plus-ECL enhanced chemiluminescence detection kit Western Lightning Enhanced Chemiluminescence Western Lightning Chemiluminescence kit Western Lightning ECL Kit Enhanced chemiluminescence (ECL) kit Western Lightning ECL Enhanced chemiluminescence kit ECL chemiluminescence kit Western Lightning

2 protocols using western lightning enhanced chemiluminescence kit ecl

Western Blot Analysis of 4-HNE Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse monoclonal 4-HNE antibody (catalog #MAB3249) was purchased from
R&D Systems (Minneapolis, MN) and goat anti-mouse horseradish
peroxidase-conjugated secondary antibody from BioRad Laboratories (Hercules,
CA). The Western Lightning enhanced chemiluminescence kit (ECL) was from Perkin
Elmer Life Sciences (Boston, MA) and Alamar Blue solution from Invitrogen (Grand
Island, NY). 4-HNE was obtained from Calbiochem (La Jolla, CA). William's Medium
E, glutamine and fetal bovine serum (FBS) were from Invitrogen Corp (Carlsbad,
CA). 4-Methylpyrazole hydrochloride was from Santa Cruz (Santa Cruz, CA). NADH,
NAD+, NADPH, NADP+, disulfiram, proteinase inhibitors and all other chemicals
were from Sigma-Aldrich (St. Louis, MO).

Zheng R., Dragomir A.C., Mishin V., Richardson J.R., Heck D.E., Laskin D.L, & Laskin J.D. (2014). Differential metabolism of 4-hydroxynonenal in liver, lung and brain of mice and rats. Toxicology and applied pharmacology, 279(1), 43-52.

+ Open protocol

+ Expand

Western Blot Analysis of Oxidative Stress Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse monoclonal 4-HNE antibody was from R&D Systems (Minneapolis, MN) and rabbit polyclonal HO-1 antibody was from Enzo Life Sciences (Farmingdale, NY). Rabbit polyclonal caveolin-1 antibody, rabbit polyclonal p38, phospho-p38, JNK, phospho-JNK, ERK1/2, phospho-ERK1/2, Akt and phospho-Akt antibodies were from Cell Signaling Technology (Beverly, MA). The DC (Detergent Compatible) protein assay kit was purchased from Bio-Rad Laboratories (Hercules, CA) and the Western Lightning enhanced chemiluminescence kit (ECL) from Perkin Elmer Life Sciences (Boston, MA). Reagents for MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) viability assays and M-MLV Reverse Transcriptase were from Promega (Madison, WI). SYBR Green Master Mix and other PCR reagents were purchased from Applied Biosystems (Foster City, CA). 4-HNE, PD 98059, SP600125 and wortmannin were from Calbiochem (La Jolla, CA). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were from Invitrogen Corp. (Carlsbad, CA). Mouse monoclonal β-actin antibody, SB203580, protease inhibitor cocktail (4-(2 aminoethyl)benzenesulfonyl fluoride, aprotinin, bestatin hydrochloride, N-(trans-epoxysuccinyl)-L-leucine 4-guanidinobutylamide, EDTA and leupeptin), methyl-β-cyclodextrin and all other chemicals were from Sigma-Aldrich (St. Louis, MO).

Zheng R., Heck D.E., Mishin V., Black A.T., Shakarjian M.P., Kong A.N., Laskin D.L, & Laskin J.D. (2014). Modulation of keratinocyte expression of antioxidants by 4-hydroxynonenal, a lipid peroxidation end product. Toxicology and applied pharmacology, 275(2), 113-121.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!