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Cd62l apc ef780

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The CD62L - APC-eF780 is a fluorochrome-conjugated monoclonal antibody that binds to the CD62L antigen, also known as L-selectin. It is used in flow cytometry applications to identify and quantify cells expressing the CD62L surface marker.

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Lab products found in correlation

Cd25 pe, by Thermo Fisher Scientific (2 mentions) Cd3 bv605, by BioLegend (2 mentions) Ccr7 pe, by BioLegend (2 mentions) Cd4 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Igd fitc, by BD (2 mentions) Pd 1 apc, by Thermo Fisher Scientific (2 mentions) Cd8 bv650, by Thermo Fisher Scientific (2 mentions) Cd45ra percp cy5, by Thermo Fisher Scientific (2 mentions) Cd19 bv450, by BD (2 mentions) Cxcr5 af488, by BioLegend (2 mentions) Hla dr efluor450, by Thermo Fisher Scientific (2 mentions) Cxcr3 fitc, by BioLegend (2 mentions) Cd27 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd127 apc, by Thermo Fisher Scientific (2 mentions) Histopaque 1077, by Merck Group (2 mentions) Lsrfortessa, by BD (2 mentions) Facsdiva software, by BD (2 mentions) Cd8 fitc, by BD (1 mentions) Cytofix cytoperm solution, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD62L (58 citations) EF780 (17 citations) CD62L-APC (9 citations)

3 protocols using cd62l apc ef780

1

Multiparametric Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells were prepared for analysis by density centrifugation using Histopaque-1077 (Sigma-Aldrich). The following antibodies were used for flow cytometry immunophenotyping: CD3 – BV605 (Biolegend, San Diego, CA, USA), CD4 – APC-eFluor780 (eBioscience, San Diego, CA,USA), CD8 – BV650 (eBioscience, San Diego, CA,USA), CD25 – PE (eBioscience, San Diego, CA,USA), CD127 – APC (eBioscience, San Diego, CA,USA), CD45RA – PerCP-Cy5.5(eBioscience, San Diego, CA,USA, CD19 – BV450 (BD Bioscience, Franklin Lakes, NJ, USA), CD27 – PE-Cy7 (eBioscience, San Diego, CA,USA, CD62L – APC-eF780 (eBioscience, San Diego, CA,USA, CXCR3 – FITC (Biolegend, San Diego, CA, USA), CXCR5 – AF488 (Biolegend, San Diego, CA, USA), CCR7 – PE (Biolegend, San Diego, CA, USA), PD-1 – APC (eBioscience, San Diego, CA,USA), HLA-DR- eFluor450 (eBioscience, San Diego, CA, USA), IgD – FITC (BD Bioscience, Franklin Lakes, NJ, USA). Flow cytometry analysis was performed on a BD LSRFortessa (BD Bioscience) with FACS Diva software (BD Bioscience) for acquisition, then analysis was performed with FlowJo software (LLC).
Thaventhiran J.E., Allen H.L., Burren O.S., Rae W., Greene D., Staples E., Zhang Z., Farmery J.H., Simeoni I., Rivers E., Maimaris J., Penkett C.J., Stephens J., Deevi S.V., Sanchis-Juan A., Gleadall N.S., Thomas M.J., Sargur R.B., Gordins P., Baxendale H.E., Brown M., Tuijnenburg P., Worth A., Hanson S., Linger R., Buckland M.S., Rayner-Matthews P.J., Gilmour K.C., Samarghitean C., Seneviratne S.L., Sansom D.M., Lynch A.G., Megy K., Ellinghaus E., Ellinghaus D., Jorgensen S.F., Karlsen T.H., Stirrups K.E., Cutler A.J., Kumararatne D.S., Chandra A., Edgar J.D., Herwadkar A., Cooper N., Grigoriadou S., Huissoon A., Goddard S., Jolles S., Schuetz C., Boschann F., Lyons P.A., Hurles M.E., Savic S., Burns S.O., Kuijpers T.W., Turro E., Ouwehand W.H., Thrasher A.J, & Smith K.G. (2020). Whole genome sequencing of a sporadic primary immunodeficiency cohort. Nature, 583(7814), 90-95.
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2

Multiparametric Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells were stained with fluorochrome-labeled monoclonal mouse anti-human antibodies (mAb) against surface antigens (CD3-Energy Coupled Dye (ECD, Beckman Coulter, clone UCHT1), CD4-Pacific Orange (BD Biosciences, clone SK3), CD8-FITC (BD Biosciences, clone HIT8a), CD19 (Invitrogen, clone SJ25-C1)/CD14 Invitrogen, clone TüK4)/Vivid-Pacific Blue, CD45RA Quantum Dots (Qdot) 655 (clone 5H3), and CD62L APC-EF780 (eBiosciences, clone DREG-56), followed by fixation/permeabilization by using cytofix/cytoperm solution (eBiosciences) and intracellular staining with mAb to IFN-γ-APC (BD Biosciences, clone B27), IL-2-phycoerythrin (PE)-Cy7 (BD Biosciences, clone MQ1-17H12), TNF Alexa Flour 700 (BD Biosciences, clone Mab11), IL-17A PerCP-Cy5.5 (eBiosciences, clone eBio64DEC17), and CD69-PE (eBiosciences, clone FN50).
Lyke K.E., Dabo A., Arama C., Diarra I., Plowe C.V., Doumbo O.K, & Sztein M.B. (2018). Long-term Maintenance of CD4 T Cell Memory Responses to Malaria Antigens in Malian Children Coinfected with Schistosoma haematobium. Frontiers in Immunology, 8, 1995.
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3

Multiparametric Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells were prepared for analysis by density centrifugation using Histopaque-1077 (Sigma-Aldrich). The following antibodies were used for flow cytometry immunophenotyping: CD3 – BV605 (Biolegend, San Diego, CA, USA), CD4 – APC-eFluor780 (eBioscience, San Diego, CA,USA), CD8 – BV650 (eBioscience, San Diego, CA,USA), CD25 – PE (eBioscience, San Diego, CA,USA), CD127 – APC (eBioscience, San Diego, CA,USA), CD45RA – PerCP-Cy5.5(eBioscience, San Diego, CA,USA, CD19 – BV450 (BD Bioscience, Franklin Lakes, NJ, USA), CD27 – PE-Cy7 (eBioscience, San Diego, CA,USA, CD62L – APC-eF780 (eBioscience, San Diego, CA,USA, CXCR3 – FITC (Biolegend, San Diego, CA, USA), CXCR5 – AF488 (Biolegend, San Diego, CA, USA), CCR7 – PE (Biolegend, San Diego, CA, USA), PD-1 – APC (eBioscience, San Diego, CA,USA), HLA-DR- eFluor450 (eBioscience, San Diego, CA, USA), IgD – FITC (BD Bioscience, Franklin Lakes, NJ, USA). Flow cytometry analysis was performed on a BD LSRFortessa (BD Bioscience) with FACS Diva software (BD Bioscience) for acquisition, then analysis was performed with FlowJo software (LLC).
Thaventhiran J.E., Allen H.L., Burren O.S., Rae W., Greene D., Staples E., Zhang Z., Farmery J.H., Simeoni I., Rivers E., Maimaris J., Penkett C.J., Stephens J., Deevi S.V., Sanchis-Juan A., Gleadall N.S., Thomas M.J., Sargur R.B., Gordins P., Baxendale H.E., Brown M., Tuijnenburg P., Worth A., Hanson S., Linger R., Buckland M.S., Rayner-Matthews P.J., Gilmour K.C., Samarghitean C., Seneviratne S.L., Sansom D.M., Lynch A.G., Megy K., Ellinghaus E., Ellinghaus D., Jorgensen S.F., Karlsen T.H., Stirrups K.E., Cutler A.J., Kumararatne D.S., Chandra A., Edgar J.D., Herwadkar A., Cooper N., Grigoriadou S., Huissoon A., Goddard S., Jolles S., Schuetz C., Boschann F., Lyons P.A., Hurles M.E., Savic S., Burns S.O., Kuijpers T.W., Turro E., Ouwehand W.H., Thrasher A.J, & Smith K.G. (2020). Whole genome sequencing of a sporadic primary immunodeficiency cohort. Nature, 583(7814), 90-95.
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