In accordance with the instructions, an EdU analysis kit (Ribobio) was applied to evaluate CaSki and SiHa cell proliferation. The cells were incubated with 50 μM EdU at 37°C, later fixed with 4% formaldehyde, and permeabilized with 0.5% Triton X-100 at room temperature for 20 minutes. The cells were later incubated with the 1 × Apollo reaction mixture. After incubating, the DNA were stained with 4ʹ, 6-diamidino2-phenylindole, and EdU-positive cells were observed under fluorescence microscopes (Carl Zeiss).
Edu analysis kit
Manufactured by RiboBio
Sourced in China
The EdU analysis kit is a laboratory tool designed to detect and quantify cell proliferation. It contains reagents and materials necessary to perform the EdU (5-ethynyl-2'-deoxyuridine) assay, which is a method for labeling and detecting newly synthesized DNA in proliferating cells.
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Lab products found in correlation
2 protocols using edu analysis kit
1
Evaluating Cell Proliferation via EdU Analysis
2
Cell Proliferation Analysis via EdU Assay
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The EdU analysis kit (RiboBio, China) was used to assess cell proliferation. ESCC cells were seeded in confocal laser cuvette ESCC plates at a density of 5 × cells/well. Next, the cells were incubated in a medium containing 50 μM EdU (C10310-1, RiboBio) for 6 h and processed according to the manufacturer’s instructions. Images were acquired under fluorescence microscopy. The average proportion of EdU-positive cells in three random field of view was analyzed.
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