After starving in serum-free media for 12 hours, 5 × 104 cells were seeded on the top chamber of transwell (6.5-µm pore; Corning, MA, USA) and incubated with ascites or TGF-β (5 μg/mL) and TGF-β antibody (final concentration 2 μg/mL) (Santa Cruz Biotechnology), while 600 µL media containing 10% FBS was added to the bottom chamber. Ten hours later, the cells remaining in the upper chamber were removed with cotton swabs and the cells on the undersurface of transwell were fixed and stained with crystal violet solution. The number of migratory cells was calculated by counting five different fields under a phase-contrast microscope of each transwell filter.
Transwell 6.5 m pore
Manufactured by Corning
Sourced in United States
The Transwell (6.5-µm pore) is a laboratory device designed for cell culture applications. It features a permeable membrane with a pore size of 6.5 micrometers, enabling the study of cell migration, barrier function, and other cellular interactions.
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Lab products found in correlation
2 protocols using transwell 6.5 m pore
1
Transwell Migration Assay for Cells
2
Transwell Migration Assay for PC3 and DU145 Cells
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A Transwell (6.5 µm pore; Corning Incorporated, Corning, NY, USA) migration assay was performed. Top chambers were seeded with 100 µl RPMI-1640 containing PC3 and DU145 cells (1×104 cells per well) and the bottom chambers were filled with 600 µl RPMI-1640, and the chambers were incubated for 24 h at 37°C under 5% CO2. After 24 h, the bottom chamber was washed with 1xPBS and was filled with 600 µl RPMI-1640 with or without 200 µM fimasartan for 6 h at 37°C. Migrated cells on the bottom side of the membrane were fixed with 4% paraformaldehyde for 30 min at room temperature and washed with PBS twice. Cell migration was evaluated through hematoxylin and eosin staining. The results were measured as the mean number of migrated cells counted using a Motic AE31 optical microscope (Motic, Xiamen, China). Data are expressed as the mean ± standard deviation from 3 independent experiments.
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