Phospho p53 ser15 9284

AMONs: Three AMON sequences and control sequences (Table 1) were synthesized and purchased from Gene Tools, LLC (Philomath, OR, USA). Temozolomide for injection was purchased Merck & Co., Inc. (Whitehouse Station, NJ, USA) and diluted in sterile water to a concentration of 2.5 mg ml⁻¹. Mouse anti-tubulin antibody (T9026) was purchased from Sigma (St Louis, MO, USA). In western blotting studies, anti-MGMT (#2739), caspase 3 (#9662), caveolin1 (#3267), rab5 (#3547), clathrin (#4796), EEA1 (#3288), APPL1 (#3858) and phospho-p53 (Ser15) (#9284) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-dynamin 2 (sc-166526) and p27 (sc-527) antibodies were purchased from Santa Cruz Biotech. (Santa Cruz, CA, USA). For immunohistochemistry, Rabbit anti-MGMT (171195-1-AP) and ki67 (sc-15402) antibodies were purchased from Proteintech (Rosemont, IL, USA) and Santa Cruz Biotech, respectively.