Plan apochromat 63 1.40 na oil diciii objective lens

To visualize intracellular proteins, immunofluorescence staining was applied using primary antibodies and appropriate fluorescent secondary antibodies. The following primary antibodies were used: Acetyl-alpha Tubulin (Lys40) mouse mAb (1:100, Thermo Fisher Scientific), α-tubulin rabbit mAb (1:100, Cell signaling technology), GM-130 rabbit mAb (1:100, Cell signaling technology), Pericentrin rabbit pAb (1:100, Abcam), Hsp60 rabbit mAb (1:100, Cell signaling technology). The following secondary antibodies were used: Alexa Fluor 594 goat anti-mouse IgG (1:200, Thermo Fisher Scientific, A-11032), Alexa Fluor 488 goat anti-rabbit IgG (1:200, Thermo Fisher Scientific, A-11008). Nuclei were stained with DAPI. The LSM710 system (Carl Zeiss) with a Plan Apochromat 63 × /1.40-NA oil DICIII objective lens (Carl Zeiss) was used to acquire images.

To visualize intracellular proteins, cells were collected, fixed with 4% PFA for 10 min and permeabilized using 0.5% saporin. The following primary antibodies were used: ant- calnexin rabbit mAb (1:100, Cell signaling technology), anti-protein disulfide isomerase (PDI) mouse mAb (1:100, Thermo Fisher Scientific). The following secondary antibodies were used: Alexa Fluor 594 goat anti-mouse IgG (1:200, Thermo Fisher Scientific, A-11032), Alexa Fluor 488 goat anti-rabbit IgG (1:200, Thermo Fisher Scientific, A-11008). Nuclei were stained with DAPI. The LSM710 system (Carl Zeiss) with a Plan Apochromat 63×/1.40-NA oil DICIII objective lens (Carl Zeiss) was used to acquire images.