Tbx ps photon detection module

A Cary
5000 (Varian) UV/vis/NIR spectrophotometer and a Fluorolog 3 spectrofluorimeter
(HORIBA Jobin-Yvon) were used to record the UV–vis absorption
or PL emission spectra, respectively. PL emission absolute QY of C-Dots
and of the luminescent nanoformulations dispersed in solution was
evaluated using a “Quanta-phi” (HORIBA Jobin-Yvon) integrating
sphere coated by Spectralons. Time-correlated single photon counting
(TCSPC) measurements were performed with a FluoroHub (HORIBA Jobin-Yvon)
to investigate fluorescence lifetime of C-Dots, bare in CHCl₃ dispersion and after their encapsulation in the PLGA nanoformulations.
A picosecond laser diode (NanoLED 375L) emitting τ ≈
80 ps pulses at a 1 MHz repetition rate was used as the excitation
source. The PL signals were detected by a picosecond photon counter
(TBX ps Photon Detection Module, HORIBA Jobin-Yvon) with a temporal
resolution of ∼200 ps.

Optical absorption spectra were recorded with a Cary 5000 (Varian) spectrophotometer. PL emission spectra were recorded by using a HORIBA Jobin-Yvon Fluorolog 3 spectrofluorimeter, equipped with a 450 W Xe-lamp and double grating excitation and emission monochromators. Time-resolved PL measurements were performed by using the time-correlated single photon counting (TCSPC) technique, with an HORIBA Jobin-Yvon FluoroHub. The experimental setup was equipped with a laser diode (NanoLED 375L) emitting ≈80 ps pulses at 375 nm with a repetition rate tunable between 10 kHz and 100 MHz. The PL was dispersed by a double grating monochromator and detected by a picosecond photon counter (TBX ps Photon Detection Module, HORIBA Jobin-Yvon) with a temporal resolution of about 200 ps. The average decay lifetime was calculated as reported in the supplementary data.