Frozen liver sections were fixed by methanol and acetone mixture (1:1) at −20 °C for 5 minutes. Immunofluorescence analyses of the examined proteins were carried out with rabbit anti-FGFR4 (8562, Cell Signaling) and mouse anti-FGF19 (MAB969, R&D Systems) antibodies. Then incubation with either Fluorescein(FITC)-conjugated anti-rabbit IgG (711-095-152, Jackson ImmunoResearch) or Rhodamine RedTM-X-conjugated anti-mouse IgG (715-295-150, Jackson ImmunoResearch) was performed. Vectrashield Mounting Medium with DAPI (H-1200, VECTOR) were used to envision cell nuclei. The negative controls, in which the primary antibodies were omitted, were included in the study (data not shown). Additionally, liver tissue structures were visualized by Mayer’s Hematoxylin staining (DAKO). Images were acquired with ZEISS Axio Imager Z2 fluorescence microscope equipped with Zen Pro 2011 acquisition program.
Rhodamine redtm x conjugated anti mouse igg
Manufactured by Jackson ImmunoResearch
Sourced in United States
Rhodamine RedTM-X-conjugated anti-mouse IgG is a secondary antibody reagent produced by Jackson ImmunoResearch. It is designed to detect and visualize mouse immunoglobulin G (IgG) in various immunochemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Mayer s hematoxylin staining, by Agilent Technologies (1 mentions)
Axioimager z2 fluorescence microscope, by Zeiss (1 mentions)
Nbp1 00858, by Novus Biologicals (1 mentions)
C apochromat 40 1.2 w, by Zeiss (1 mentions)
Axiovert 100m, by Zeiss (1 mentions)
Lsm 510, by Zeiss (1 mentions)
Ab54790, by Abcam (1 mentions)
Alexa fluor 488 conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
2 protocols using rhodamine redtm x conjugated anti mouse igg
1
Immunofluorescence Analysis of FGFR4 and FGF19
2
Immunofluorescence Labeling of FFA4 and β-Arrestin 2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were labeled with antibodies against FFA4 (NBP1-00858, 1:500, Novus Biologicals, LLC, CO, USA) and β-arrestin 2 (ab54790, 1:500, Abcam, Cambridge, MA, USA). The secondary antibody used was Alexa Fluor 488-conjugated anti-rabbit IgG (1:1000, Jackson ImmunoResearch, West Grove, PA, USA) and Rhodamine RedTM-X-conjugated anti-mouse IgG (1:1000, Jackson ImmunoResearch, West Grove, PA, USA). A confocal scanning module (LSM510, Carl Zeiss, Germany) mounted on a fluorescence microscope (AXIOVERT 100 M, Carl Zeiss, Germany) and a C-Apochromat 40×/1.2 W (Carl Zeiss, Germany) was used34 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!