Approximately 2 weeks before the study day, fasting serum was withdrawn to screen for factors that might have an impact on testosterone production. Dehydroepiandrosterone sulfate (DHEAS) with catalog number L2KDS2 was measured using a solid-phase competitive chemiluminescent enzyme immunoassay with an Immulite 2000 XPi system from Siemens Healthcare diagnostics (The Hague, The Netherlands). Total 25-hydroxyvitamin D (catalog number 05894913190) was measured using ECLIA with an E170 module of Modular Analytics from Roche Diagnostics. Interleukin 6 (catalog number SS600B) and tumor necrosis factor-α (TNF-α) (catalog number SSTA00D) were measured by ELISA from R&D Systems. High-sensitivity C reactive protein (hsCRP) with catalog number 04628918190 was determined by a particle-enhanced turbidimetric assay using Cobas Integra 800 from Roche Diagnostics.
Immulite 2000 xpi system
Manufactured by Siemens
Sourced in United States, Germany
The Immulite 2000 XPi system is an automated immunoassay analyzer designed for clinical laboratory use. It is capable of performing a variety of immunoassay tests to aid in the diagnosis and monitoring of various medical conditions.
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Lab products found in correlation
9 protocols using immulite 2000 xpi system
1
Serum Biomarker Evaluation Protocol
2
IgE and Antithyroid Antibodies Quantification
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Total IgE and circulating antithyroid antibodies (ATAs) levels in serum were analyzed by a chemiluminescence immunoassay technique using the IMMULITE 2000 XPi System (Siemens).
3
Assessing Metabolic Parameters and Insulin Resistance
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Blood samples to measure biochemical parameters were collected from the antecubital vein of each participant after an overnight fast. Glucose, total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C), HDL-C, and creatinine levels were determined using an auto-analyser (Hitachi 747; Hitachi, Tokyo, Japan), and fasting plasma insulin levels were determined by radioimmunoassay (Immulite 2000 XPi system; Siemens, Erlangen, Germany). Insulin resistance was assessed using the homeostasis model assessment insulin resistance (HOMA-IR) index, which was calculated as follows: [fasting glucose (mg/dL) × fasting insulin (μIU/mL)]/405 [22 (link)]. The glomerular filtration rate was estimated by the re-expressed Modification of Diet in Renal Disease study equation using calibrated serum creatinine values [23 (link)]; the formula used for eGFR was as follows: 175 × (serum creatinine concentration)−1.154 × (age)−0.203.
4
Measuring Serum hCG Levels Using Immulite 2000 XPi
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hCG was analyzed in serum using a solid-phase two-site chemiluminescent immunometric assay, calibrated against WHO 3rd IS 75/537, on an Immulite 2000 XPi system (Siemens Healthcare Diagnostics, Deerfield, IL, USA). The Siemens assay detects serum intact hCG, hyperglycosylated hCG, serum nicked hCG, serum nicked hyperglycosylated hCG, serum asialo hCG, serum hCG free β-subunit and serum nicked hCG β [25 (link)]. The inter assay coefficient of variation was 8.0, 6.3 and 5.1 % at the concentration of 9.7, 53.1 and 821.5 IU/L, respectively. Although the Immulite 2000 is considered as one of the best assays for total hCG, it should be noted that the reference ranges in this paper are assay specific and do not correspond with hCG values obtained from different assays [26 ].
5
Quantitative hCG Immunoassay Protocol
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Total hCG was measured in serum using a solid-phase two-site chemiluminiscent immunometric assay, calibrated against WHO 3rd IS 75/537, on an Immulite 2000 XPi system (Siemens Healthcare Diagnostics, Deerfield, IL, USA). The assay detects serum intact hCG, hyperglycosated hCG, serum nicked hCG, serum nicked hyperglycosated hCG, serum asialo hCG, serum hCG free β-subunit and serum nicked hCG β. hCG concentrations were transformed to standard deviation scores adjusted to gestational age at blood sampling [19 (link)].
6
Basophil FcεRI Expression Evaluation
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All complementary tests were common to the cohort. Baseline tests included total IgE, FCεRI, ANA, IgG anti-thyroid peroxidase (TPO), tryptase, and D-dimer. FcεRI expression in blood basophils was measured by flow cytometry. Standard procedures were followed to perform flow cytometry analyses. 12 FcεRI expression in basophils was assessed by mean fluorescence intensity (MFI). To ensure consistency of analysis, the same investigator processed and analysed all samples. Baseline total IgE in serum and IgG anti-TPO levels in serum were analysed by a chemiluminescence immunoassay with IMMULITE 2000 XPi System (Siemens, Munich, Germany). All other laboratory values were measured by automated analysers in the local central laboratory.
7
Biomarker Profiling of Cholesterol, Inflammation, and Hormones
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On the study day, total cholesterol, triglycerides, and high-density lipoprotein cholesterol were measured on the Roche Cobas 8000 Modular. Approximately 2 weeks before the study day, fasting serum was withdrawn to screen for baseline factors. Dehydroepiandrosterone sulfate (catalog #L2KDS2) was measured using a solid-phase competitive chemiluminescent enzyme immunoassay with an Immulite 2000 XPi system from Siemens Healthcare diagnostics (The Hague, The Netherlands). High-sensitivity C-reactive protein (hsCRP) (catalog #04628918190) was determined by a particle-enhanced turbidimetric assay using Cobas Integra 800 from Roche Diagnostics. Interleukin 6 (catalog #SS600B) and TNF-α (catalog #SSTA00D) were measured by ELISA from R&D Systems. All measurements were performed at the Department of Clinical Chemistry and Laboratory Medicine of the Leiden University Medical Center in The Netherlands.
8
Gestational Age Standardization of hCG
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Total hCG was measured in first available samples (median gestational age, 14.4 weeks; 95% range, 10.1–26.1 weeks) using a solid‐phase two‐site chemiluminescence immunometric assay, calibrated against World Health Organization Third International Standard 75/537, on an Immulite 2000 XPi system (Siemens Healthcare Diagnostics, Deerfield, IL, USA). The assay detects serum intact hCG, hyperglycosylated hCG, serum nicked hCG, serum nicked hyperglycosylated hCG, serum asialo hCG, serum hCG free β‐subunit and serum nicked hCG β. The intra‐assay variation coefficients were 8.0%, 6.3% and 5.1% at concentrations of 9.7, 53.1 and 821.5 IU/L, respectively. hCG concentration was standardized by calculating SD scores adjusted for gestational age at blood sampling, as described previously22 .
9
Autoimmune Serum and Plasma Reactivity
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Total IgE and levels of circulating anti-TPO and anti-Tg antibodies were analyzed in serum by chemiluminescence immunoassay technique using the IMMULITE 2000 XPi System (Siemens, Munich, Germany). The ASST was performed in CSU patients as previously described. 14 Briefly, venous blood was taken at the initial evaluation, and samples were centrifuged at 2500 rpm for 10 minutes and the serum separated. For the APST, citrated blood was centrifuged at room temperature to separate the plasma. Afterwards, patients received intradermal injections of 50 µL of fresh undiluted autologous serum and 50 μL of autologous plasma on the volar forearm.
Similar volumes of 0.9% NaCl saline and 100 mg/mL histamine were used as negative and positive controls, respectively. A positive ASST/APST was considered when the diameter of serum-induced wheal was >1.5mm compared to the saline-induced response at 30 minutes.
Similar volumes of 0.9% NaCl saline and 100 mg/mL histamine were used as negative and positive controls, respectively. A positive ASST/APST was considered when the diameter of serum-induced wheal was >1.5mm compared to the saline-induced response at 30 minutes.
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