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Lipidtox staining

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LipidTOX Staining is a fluorescent staining solution for the detection of neutral lipids and lipid droplets in cells. It provides a sensitive and specific way to visualize and quantify the accumulation of lipids within cells using fluorescence microscopy or flow cytometry.

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Lab products found in correlation

Hoechst staining, by Thermo Fisher Scientific (2 mentions) Lipidtox neutral lipid stain, by Thermo Fisher Scientific (2 mentions) Rosiglitazone, by Merck Group (1 mentions) Recombinant tgf β1, by R&D Systems (1 mentions) Palmitate, by Merck Group (1 mentions) Oil red o staining, by Thermo Fisher Scientific (1 mentions) Mtt assay, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Oil Red O and HCS LipidTOX Green neutral lipid staining LipidTOX Deep Red staining LipidTOX

4 protocols using lipidtox staining

1

Visualizing Lipids in Skin Organoids

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LipidTOX Staining (Invitrogen) was performed to visualize sebaceous glands and lipid-rich adipocytes. Cryosections of skin organoids were incubated with LipidTOX neutral lipid stain diluted at a ratio of 1:200 in 1X PBS for 30 min at RT, followed by Hoechst staining (1:2000; Invitrogen) for 1 min at RT to visualize nuclei.
Lee J., Rabbani C.C., Gao H., Steinhart M.R., Woodruff B.M., Pflum Z.E., Kim A., Heller S., Liu Y., Shipchandler T.Z, & Koehler K.R. (2020). Hair-bearing human skin generated entirely from pluripotent stem cells. Nature, 582(7812), 399-404.
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2

Visualizing Lipids in Skin Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
LipidTOX Staining (Invitrogen) was performed to visualize sebaceous glands and lipid-rich adipocytes. Cryosections of skin organoids were incubated with LipidTOX neutral lipid stain diluted at a ratio of 1:200 in 1X PBS for 30 min at RT, followed by Hoechst staining (1:2000; Invitrogen) for 1 min at RT to visualize nuclei.
Lee J., Rabbani C.C., Gao H., Steinhart M.R., Woodruff B.M., Pflum Z.E., Kim A., Heller S., Liu Y., Shipchandler T.Z, & Koehler K.R. (2020). Hair-bearing human skin generated entirely from pluripotent stem cells. Nature, 582(7812), 399-404.
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3

Lung Fibroblasts and IPF Pathogenesis

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Human lung tissues and primary fibroblasts from non-IPF donors and IPF patients undergoing lung transplantation were obtained from the Giessen biobank. The study protocol (AZ31/93) was approved by the ethics committee of the University of Giessen that conforms to the principles outlined in the declaration of Helsinki. Tissues were used for RNA extraction, paraffin embedding followed by immunohistochemistry, or cryoembedding followed by LipidTOX staining (Thermo Fischer Scientific). Human lung fibroblasts were starved for 24 hr and then treated with 1 ng/mL recombinant TGFβ1 (R&D Systems) and/or 20 μM rosiglitazone (Sigma-Aldrich). Cells were harvested after 72 hr for RNA extraction and gene expression analysis.
Agha E.E., Moiseenko A., Kheirollahi V., De Langhe S., Crnkovic S., Kwapiszewska G., Kosanovic D., Schwind F., Schermuly R.T., Henneke I., MacKenzie B., Quantius J., Herold S., Ntokou A., Ahlbrecht K., Morty R.E., Günther A., Seeger W, & Bellusci S. (2016). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell stem cell, 20(2), 261-273.e3.
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4

Lipid Accumulation and Viability in C2C12 Cells

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C2C12 cells were cultured for 24 h in a medium supplemented with BSA alone (control), or BSA-conjugated oleate (500 μM) or palmitate (300–500 μM) (Merck), ± TRAIL (1; 10; 100 ng/mL). Cell viability was assessed with the MTT assay (Alfa Aesar; #L11939; Thermo Fisher Scientific). Total intracellular lipid content was evaluated and visualized by Oil Red O staining and LipidTOX staining (Thermo Fisher Scientific).
Toffoli B., Tonon F., Tisato V., Zauli G., Secchiero P., Fabris B, & Bernardi S. (2021). TRAIL/DR5 pathway promotes AKT phosphorylation, skeletal muscle differentiation, and glucose uptake. Cell Death & Disease, 12(12), 1089.
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