Total protein was lysed with RIPA buffer (KeyGEN, Biotech), and the protein concentration was determined using a BCA Protein Assay Kit (ZJ101, EpiZyme). Total protein was separated by SDS‒PAGE gel electrophoresis and transferred onto PVDF membrane (Millipore, USA). Membranes were blocked in 5 % skim milk for 1 h and incubated overnight with primary antibodies at 4°C. Then, membranes were incubated with secondary antibody and visualized. The primary antibodies used were anti-AEBP1 (1: 1000, sc-271374, Santa Zruz, USA), anti-GAPDH (1: 10000, AC003, Abclonal, China), anti-BMP4 (1: 2000, ab124715, Abcam, UK), anti-E-cad (1: 2000, 14472, CST, USA), anti-N-cad (1: 1000, 13116, CST, USA), anti-vimentin (1: 1000, 3932, CST, USA), anti-TWIST1 (1: 1000, 90445, CST, USA), anti-ZEB2 (1: 1000, ab223688, Abcam, UK), anti-Smad1/5/9 (1: 1000, ab300164, Abcam, UK), and anti-p-Smad1/5/9 (1: 1000, AP0850, Abclonal).
Ab223688
Manufactured by Abcam
Sourced in United Kingdom
Ab223688 is a laboratory product manufactured by Abcam. It is a piece of lab equipment, but the core function of this product is not available in a detailed, unbiased, and factual description without interpretation or extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Ab124715, by Abcam (1 mentions)
Bca protein assay kit, by Ipsen (1 mentions)
Ac003, by ABclonal (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ripa buffer, by Keygen Biotech (1 mentions)
Ab2213, by Abcam (1 mentions)
Ab137321, by Abcam (1 mentions)
Ab226942, by Abcam (1 mentions)
Ab92337, by Abcam (1 mentions)
Ab1416, by Abcam (1 mentions)
Ab32143, by Abcam (1 mentions)
Sc 47778, by Santa Cruz Biotechnology (1 mentions)
Ab46154, by Abcam (1 mentions)
Ha 2367, by Cell Signaling Technology (1 mentions)
2 protocols using ab223688
1
Western Blot Analysis of EMT Markers
2
Evaluating EMT Pathway Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human p-CMV-ICAM-1 vector was purchased from sinobiological, pc-DNA3-SRC vector was purchased from Addgene, and p-CMV6-c-MET vector was purchased from origene. HGF (100-39H) was purchased from Peprotech. SU11274 (c-MET inhibitor), WP1066 (573097), and PP2 (529573) were obtained from Calbiochem. Antibodies specific for CDH2 (#610920) was purchased from BD Biosciences (San Jose, CA, USA). Antibodies specific for Snail(#3879), Slug (#9585), TWIST (#46702),SRC (#2108), p-SRC (#2105), MET (#3127), p-MET (#3077), and HA (#2367) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies specific for Vim (ab137321), CDH1 (ab1416), PDGFB (ab23914), VEGFA (ab46154), FGF2 (ab92337), STAT3 (ab226942), p-STAT3 (ab32143), ZEB1 (ab223688) and ICAM-1 (ab2213) was obtained from Abcam (Cambridge, UK). The monoclonal antibodies specific for β-Actin (SC-47778) was purchased from Santa Cruz. The monoclonal antibodies specific for FLAG (F-3165) was purchased from Sigma. The polyclonal antibodies specific for p-ICAM-1 (CSB-PA000547) was purchased from CUSABIO TECHNOLOGY.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!