2 mce

Manufactured by Merck Group

Sourced in United States

2-mce is a type of laboratory equipment used for analytical and research purposes. It is a chemical compound commonly used as a reagent or intermediate in various scientific experiments and analyses. The core function of 2-mce is to serve as a tool for researchers and scientists in their investigations, but a more detailed description without interpretation or extrapolation cannot be provided.

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Lab products found in correlation

2 protocols using 2 mce

iPSC Cultivation and Dopaminergic Differentiation

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iPSCs were cultivated in the growth medium containing KnockOut DMEM, 15% KnockOut Serum Replacement, GlutaMAX-I, 0.1 mM NEAA, 1% penicillin-streptomycin (all from Thermo Fisher Scientific, Waltham, MA, USA), 0.1 mM 2-mce (Sigma-Aldrich, Darmstadt, Germany), and 10 ng/mL bFGF (SCI Store, Moscow, Russia) onto a gelatin-coated plate with mouse embryonic fibroblasts (MEF) treated with Mitomycin C from Streptomyces caespitosus (Sigma-Aldrich, Darmstadt, Germany). For DA differentiation, iPSCs were passaged onto Matrigel-GFR (Corning, New York, NY, USA) in Essential 8 Medium (Thermo Fisher Scientific, Waltham, MA, USA). iPSCs were passaged 2 times a week at a ratio of 1:8–1:10 with the addition of 2 μg/mL Thiazovivin (Sigma-Aldrich, Darmstadt, Germany). Dissociation of iPSC colonies was carried out using TrypLE (Thermo Fisher Scientific, Waltham, MA, USA). Cells were cultured in a CO₂ incubator (37 °C, 5% CO₂).

Grigor’eva E.V., Kopytova A.E., Yarkova E.S., Pavlova S.V., Sorogina D.A., Malakhova A.A., Malankhanova T.B., Baydakova G.V., Zakharova E.Y., Medvedev S.P., Pchelina S.N, & Zakian S.M. (2023). Biochemical Characteristics of iPSC-Derived Dopaminergic Neurons from N370S GBA Variant Carriers with and without Parkinson’s Disease. International Journal of Molecular Sciences, 24(5), 4437.

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Synthesis of Indium-based Nanocrystals

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Indium acetate [In(OAc)₃, 99.99%], oleic acid (OA, technical grade, 90%), dioctylamine (DOA, Aldrich, ≥97%), 1-octadecene (ODE, 90%), N, N-dimethylformamide (DMF, 99.8% anhydrous), octane (99% anhydrous), 1-butanol (BuOH, 99.8% anhydrous), toluene (Tol, 99.8% anhydrous), ammonium acetate (AA, 99.999%), 2-MCE (99%), MTA (98%) were purchased from Sigma-Aldrich. Tris(trimethylsilyl)arsine [(TMSi)₃As] was purchased from Dockweiler Chemicals Inc. Indium (III) bromide (InBr₃, 99.999% anhydrous) was purchased from Strem Chemicals, Inc. All the mentioned chemicals were used as received.

Vafaie M., Morteza Najarian A., Xu J., Richter L.J., Li R., Zhang Y., Imran M., Xia P., Ban H.W., Levina L., Singh A., Meitzner J., Pattantyus-Abraham A.G., García de Arquer F.P, & Sargent E.H. (2023). Molecular surface programming of rectifying junctions between InAs colloidal quantum dot solids. Proceedings of the National Academy of Sciences of the United States of America, 120(41), e2305327120.

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