Blood samples were collected in 2ml K3EDTA tubes (ThermoFisher Scientific, Massachusetts, USA). On arrival to the laboratory, samples were centrifuged at 1500g for 15 minutes at room temperature, and plasma removed and frozen at -80°C until processing for cytokine measurement. All candidate proteins were measured utilizing Magnetic Luminex® Performance Multiplex Assay (R&D Systems Inc., Minneapolis, USA) using the customised 7-plex Human High Sensitivity Cytokine Premixed kit (R&D, FCSTM14). From the cytokines available for “high-sensitivity” measurement, we chose five cytokines known to be stimulated by IFN-α (interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α) (Taylor and Grossberg, 1998 (link))) and two that are inhibited (interleukin-7 (IL-7) (Su et al, 1997 (link)) and interleukin-17A (IL-17A) (Cui et al, 2014 (link))). All samples were assayed according to the manufacturer’s protocol, and the results analyzed using SoftMax Pro V4.8 (full details in Supplemental Materials ). IFN-γ levels were below detection limit for most samples, therefore were not analysed further.
Magnetic luminex performance multiplex assay
Manufactured by R&D Systems
Sourced in United States
The Magnetic Luminex® Performance Multiplex Assay is a lab equipment product that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The assay utilizes color-coded magnetic beads and laser-based detection technology to provide a versatile and efficient platform for protein and cytokine analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using magnetic luminex performance multiplex assay
1
Multiplex Cytokine Profiling in Plasma
2
Quantifying Cytokine Profiles in Plasma Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected in 2 ml K3EDTA tubes (ThermoFisher Scientific). On arrival to the laboratory, samples were centrifuged at 1500 g for 15 min at room temperature and plasma were removed and frozen at −80 °C until processing for cytokine measurement. All candidate proteins were measured using Magnetic Luminex Performance Multiplex Assay (R&D Systems, Minneapolis, USA), using the customized 7-plex Human High Sensitivity Cytokine Premixed kit (R&D, FCSTM14). From the cytokines available for ‘high-sensitivity' measurement, we chose five cytokines known to be stimulated by IFN-α (IL-1β, IL-2, IL-6, IFN-γ, and TNF-α (Taylor and Grossberg, 1998 )) and two that are inhibited (IL-7 (Su et al, 1997 (link)) and IL-17A (Cui et al, 2014 (link))). All samples were assayed according to the manufacturer's protocol and the results analyzed using SoftMax Pro V4.8 (full details in Supplementary Materials ). IFN-γ levels were below detection limit for most samples and therefore were not analyzed further.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!