Tilapia skin was provided by Hailisheng Group Co., Ltd. (Zhoushan, Zhejiang, China). L. rhamnosus ATCC7469 was provided by Shanghai Luwei Microbial Sci. & Tech. Co., Ltd. (Shanghai, China). MRS broth and MRS agar were obtained from Qingdao Hope Bio-Technology CO., Ltd. (Tsingtao, China). Dispase (50,000 U/g), alkaline proteinase (200,000 U/g), papain (100,000 U/g), compound proteinase (100,000 U/g), trypsin (250,000 U/g), BCA protein assay kit, β-GAL assay kit, and LDH assay kit were obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). The other reagents and chemicals were analytical grade.
Mrs agar
Manufactured by Hopebio
Sourced in China
MRS agar is a microbiological growth medium used for the isolation and cultivation of lactic acid bacteria. It provides nutrients and growth factors required for the optimal growth of lactobacilli and other lactic acid-producing microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
β gal assay kit, by Solarbio (2 mentions)
Trypsin, by Solarbio (2 mentions)
Bca protein assay kit, by Solarbio (2 mentions)
Papain, by Solarbio (2 mentions)
Ldh assay kit, by Solarbio (2 mentions)
Mrs broth, by Hopebio (2 mentions)
Dispase, by Solarbio (1 mentions)
Compound proteinase, by Solarbio (1 mentions)
Alkaline proteinase, by Solarbio (1 mentions)
Protamex, by Solarbio (1 mentions)
Alcalase, by Solarbio (1 mentions)
Neutrase, by Solarbio (1 mentions)
Mrs broth mrsb, by Hopebio (1 mentions)
Plate count agar, by Hopebio (1 mentions)
Violet red bile agar, by Hopebio (1 mentions)
Bismuth sulfite agar, by Hopebio (1 mentions)
Macconkey agar, by Hopebio (1 mentions)
5 protocols using mrs agar
1
Tilapia Skin Bioactive Compounds Extraction
2
Enzymatic Hydrolysis of Antarctic Krill
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Defatted Antarctic krill powder was provided by Zhejiang Hailisheng Biotechnology Co., Ltd. (Zhoushan, China), with a proximate composition as follows: moisture (8.29%), protein (78.33%), fat (1.17%), sugar (0.84%), and ash (4.62%). Neutrase (5 × 104 U/g), Alcalase (2 × 105 U/g), Papain (1 × 105 U/g), Trypsin (2.5 × 105 U/g), Protamex (1 × 105 U/g), the BCA protein assay kit, the β-GAL assay kit, and the LDH assay kit were obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). L. rhamnosus ATCC7469 was provided by Shanghai Luwei Microbial Sci. &Tech. Co., Ltd. (Shanghai, China). MRS broth and MRS agar were purchased from Qingdao Hope Bio-Technology Co., Ltd. (Tsingtao, China). The other reagents and chemicals were of analytical grade.
3
Culturing L. intestinalis (ATCC49335)
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The L. intestinalis (ATCC49335) used in this study was purchased by Beijing Beina Chuanglian Biotechnology Research Institute (Beijing, China). Unless otherwise stated, bacterial strains were grown in MRS Broth (MRSB) (Qingdao Hope Bio-technology Corporation Ltd.) or on MRS Agar (MRSA) plates at 37°C.
4
Bacterial Enumeration in Sauerkraut
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The bacterial counts of sauerkraut were determined by the plate counting method. The sauerkraut brine was serially diluted, and 1.0 mL was pipetted into the corresponding agar medium plate for counting. LAB counts were detected on de Man Rogosa Sharpe (MRS) agar (Hopebio, Qingdao, China) after incubation for 48 h at 37 °C; aerobic counts were detected on Plate Count Agar (PCA) (Hopebio, Qingdao, China) after incubation for 48 h at 37 °C; and Enterobacteriaceae counts were detected on Violet Red Bile Agar (VRBA) (Hopebio, Qingdao, China) after incubation for 48 h at 37 °C.
5
Enumeration of Cecal Microbiome
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Approximately 0.2 g of aseptically removed cecal contents were diluted in 2 mL of sterilized saline (154 mmol/L), and then three 10-fold serial dilutions were made from the diluted cecal contents (10−3, 10−4 and 10−5 for Salmonella; 10−4, 10−5 and 10−6 for Escherichia coli and Lactobacillus). A 100 μL portion of the last three dilutions were then spread evenly onto plates. Escherichia coli colonies were enumerated on MacConkey agar (Qingdao Hope Bio-Technology Co. Ltd., Qingdao, Shandong, China) at 37 °C for 24 h. Lactobacillus were enumerated on MRS agar (Qingdao Hope Bio-Technology Co. Ltd., Qingdao, Shandong, China) medium at 37 °C for 48 h. Salmonella colonies were determined on Bismuth sulfite agar (Qingdao Hope Bio-Technology Co. Ltd., Qingdao, Shandong, China) and incubated at 37 °C for 24 h. All plates with countable colonies were enumerated and averaged to express log CFU (Colony-Forming Units) per gram of cecal content.
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