All restriction enzymes were obtained from New England Biolabs. Chemicals were obtained from SIGMA-Aldrich unless otherwise specified. TTX, bicuculline, and strychnine were obtained from TOCRIS Bioscience. SynGAP antibodies used included SynGAP (Sigma Aldrich SAB2501893), SynGAP-α1 (Santa Cruz sc-8572), pan-SynGAP (Thermo scientific PA-1-046), as well as isoform-specific SynGAP antibodies previously developed in the Richard Huganir laboratory: SynGAP-α1 JH2469, SynGAP-α2 JH7265, SynGAP-β JH7206, and SynGAP-γ JH7366 (17 (link)). Other antibodies used included Phosphorylated ERK (Cell Signaling Technology 9106) and total ERK (Cell Signaling Technology 9102) and Tubulin (Sigma Aldrich T5168). DNA sequencing was performed at the Johns Hopkins University School of Medicine Sequencing Facility.
Syngap
Manufactured by Thermo Fisher Scientific
SynGAP is a laboratory equipment product offered by Thermo Fisher Scientific. It is a specialized tool used for the analysis and study of synaptic protein complexes. The core function of SynGAP is to facilitate the measurement and characterization of synaptic proteins and their interactions within a controlled laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Total erk, by Cell Signaling Technology (1 mentions)
Bicuculline, by Bio-Techne (1 mentions)
Strychnine, by Bio-Techne (1 mentions)
Phosphorylated erk, by Cell Signaling Technology (1 mentions)
Psd 95, by Thermo Fisher Scientific (1 mentions)
Brain derived neurotrophic factor (bdnf), by Merck Group (1 mentions)
Manganese 2 chloride tetrahydrate, by Merck Group (1 mentions)
Brain derived neurotrophic factor (bdnf), by Abcam (1 mentions)
β actin, by Thermo Fisher Scientific (1 mentions)
Lactate dehydrogenase ldh kit, by Beyotime (1 mentions)
2 protocols using syngap
1
Characterization of SynGAP Isoform-Specific Antibodies
2
Investigating Synaptic Dynamics in α-Synuclein Pathology
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Manganese (II) chloride tetrahydrate, BDNF, and DuoLink proximity ligation assay (PLA) reagent were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The pGCSIL-GFP-α-Syn small hairpin RNA (shRNA)10 (link) was purchased from Shanghai GeneChem Co. Ltd. (Shanghai, China). A Cell Counting Kit-8 (CCK-8) assay kit and lactate dehydrogenase (LDH) kit were purchased from Beyotime Biotech Co. Ltd. (China). α-Syn primary antibody was obtained from Thermo Fisher Scientific. PSD95, SynGAP, β-actin, phospho-CaMKII (Thr286), CaMKII-α, NMDA receptor 2A (GluN2A), NMDA receptor 2B (GluN2B), Fyn, phospho-NMDA receptor 2A (GluN2A) (Tyr1246), and phospho-NMDA receptor 2B (GluN2B) (Tyr1472) primary antibodies were purchased from Cell Signaling Technology. TrkB, AKT, and AKT-phospho-S473 primary antibodies were purchased from Proteintech Biotech Co. Ltd. (China). BDNF and phospho-Fyn (Y530) were purchased from Abcam Ltd. (Hong Kong). Secondary antibodies including horseradish peroxidase (HRP)-conjugated anti-rabbit and HRP-conjugated anti-mouse were purchased from Abcam Ltd. (Hong Kong).
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