Embryos, embryonic or postnatal hearts were whole-mount stained with X-gal as described (Qu et al., 2015 (link)). In short, freshly dissected embryos or hearts were fixed in glutaraldehyde, and whole embryos or hearts were stained overnight and processed for paraffin embedding. X-gal staining was performed on frozen sections as described (Qu et al., 2010 (link)). Briefly, cryoprotected embryos were embedded in OCT (Tissue Tek) and cryosectioned, stained for β-galactosidase (β-gal) activity, and then immuno-stained with primary antibody goat anti-Tie2 (R&D Systems) and fluorescently labeled secondary antibody.
Goat anti tie2
Manufactured by R&D Systems
Sourced in United States
Goat anti-Tie2 is a laboratory reagent that can be used to detect and measure the Tie2 protein. Tie2 is a receptor tyrosine kinase that plays a role in angiogenesis and vascular development. The antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and ELISA, to study the expression and localization of Tie2 in biological samples.
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Lab products found in correlation
Rabbit anti mouse peroxidase, by Merck Group (1 mentions)
Goat anti rabbit peroxidase, by Merck Group (1 mentions)
Rabbit anti tie2, by Santa Cruz Biotechnology (1 mentions)
Goat anti histone h3, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti gβ, by Santa Cruz Biotechnology (1 mentions)
Mouse anti γ tubulin, by Merck Group (1 mentions)
Mouse anti ptyrosine 4g10, by Merck Group (1 mentions)
Donkey anti goat fitc, by OriGene (1 mentions)
D glucose, by Merck Group (1 mentions)
2 protocols using goat anti tie2
1
Whole-mount X-gal staining of embryos and hearts
2
Quantification of Angiopoietin-2 Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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soluble Tie2-Fc chimera human (sTie2) (C-68028, Promokine, Germany, 5 µg/mL), D-Glucose (G7021, Sigma, Germany 5 mM or 30 mM), gelatin from porcine skin (48720, Fluka, Bucharest, Romania, 1% solution in PBS) was used to coat cell culture and assay plates. The antibodies used were: mouse anti-NDPK-B (Kamiya Biomedicals, Seattle, WA, USA; MC-412); mouse anti-Ang2 (sc-74403, Santa Cruz Biotechnology, Dallas, TX, USA); rabbit anti-Tie2 (Santa Cruz Biotechnology, Dallas, TX, USA, sc-324); mouse anti-pTyrosine 4G10 (Millipore, Burlington, MA, USA; 05-321); mouse anti-γ-tubulin (Sigma-Aldrich, St. Louis, MO, USA; T6557); rabbit anti-Gβ (Santa Cruz Biotechnology, Dallas, TX, USA; sc-378); goat anti-Histone H3 (Santa Cruz Biotechnology, Dallas, TX, USA; sc-8654); Isolectin-TRITC (Sigma-Aldrich, St. Louis, MO, USA; L5264); goat anti-Tie2 (R&D Systems, Minneapolis, MN, USA; AF762); mouse anti-Tie2/TEK clone AB33 (Merck, Darmstadt, Germany; 05-584) rabbit anti-goat peroxidase (Sigma-Aldrich, St. Louis, MO, USA; A8919); rabbit anti-mouse peroxidase (Sigma-Aldrich, St. Louis, MO, USA; A9044); goat anti-rabbit peroxidase (Sigma-Aldrich, St. Louis, MO, USA; A9169); donkey anti-goat-FITC (Acris Antibodies, Hiddehausen, Germany; R1254F); swine anti-rabbit-FITC (Dako Agilent Technologies, Santa Clara, CA, USA; F0205)
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