Anti c3c antibody
The Anti-C3c antibody is a laboratory product designed to detect and measure the presence of the C3c component of the complement system. It is a specific and sensitive tool used in various research and clinical applications related to the complement cascade and immune system function.
3 protocols using anti c3c antibody
Cofactor Activity Assay of Complement Factor H
Uromodulin Modulates CFH-C3b Binding
The CFH‐mediated C3b inactivation assay, including CFH (1 μg), factor I (50 ng), C3b (3 μg) and uromodulin (0, 4, 8 and 16 μg), was conducted in a final volume of 20 μl and incubated at 37°C for 30 min. Experiments conducted in the absence of CFH were performed as negative controls. After incubation, the samples were heated for 5 min at 95°C in reducing buffer containing β‐mercaptoethanol and run on a 10% SDS‐PAGE gel. C3b and its cleavage products were detected by western blotting using a polyclonal anti‐C3c antibody (Dako Cytomation, Carpinteria, CA, USA).
Ex Vivo Complement Activation Post-TBI
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